保肾口服液对IgA肾病小鼠B细胞增殖和T细胞分泌IL-4的影响  

INFLUNENCE OF BAOSHEN ORAL LIQUOR ON B CELL PROLIFERATION AND IL-4 SECRETED BY T CELL OF MICE WITH IgA NEPHROPATHY

作　　者：陈玉春[1] 阮诗玮[2] 王碧英[1] 王伟[2] 

机构地区：[1]福建省中医药研究院,福州350003 [2]福建中医学院附属人民医院,福州350004

出　　处：《中国中医药科技》2004年第6期331-333,共3页Chinese Journal of Traditional Medical Science and Technology

基　　金：福建省卫生厅中医药重点资助课题No.0 70 2

摘　　要：目的 :观察保肾口服液对IgA肾病 (IgAN)小鼠体液免疫功能的影响。方法 :通过腹腔注射交联葡聚糖凝胶 (SephadexG - 2 0 0 )预免疫和右旋糖酐 (Dextran)免疫的方法建造IgAN模型。测定LPS刺激的小鼠脾B淋巴细胞3 H -TdR掺入和PWM刺激的小鼠脾T淋巴细胞合成和分泌白细胞介素 - 4 (IL - 4 )水平。结果 :低、中、高 3种浓度的保肾口服液均能明显或显著促进IgAN小鼠自发性和脂多糖 (LPS)刺激的B淋巴细胞3 H -TdR掺入 ;且有剂量依赖关系。保肾口服液能明显调低T淋巴细胞分泌IL - 4的水平 ,提前给药有显著的调低作用。结论 :保肾口服液显著保进IgAN小鼠B淋巴细胞活化和增殖 ,增强其体液免疫功能 ,调低T淋巴细胞分泌IL - 4水平。Objective:To obserre the effects of baoshen(Kidney protection) oral liquor on humoral immunity of IgA nephropathy (IgAN).Method:The model of IgAN mice was made by preimmune by means of peritoneal injection iof sephadex G-200 and immune by means of peritoneal injection of Dextran.Level of functional humoral immunity was indcated by measuring 3 H-TdR incorporation of LPS-stimulated murine splenic B lymphocytes and level of interleukin-4 (IL-4) syntheszed and secreted by PWM-stimulated murine splenic T lymphocytes.Results:Low,medium and high concentration baoshen oral liquior were all able to notable significantly enhance 3 H-TdR incorporation of spontaneous and LPS-stimulated IgAN murine B lymphocytes in a dose-dependent manner.On the other hand,baoshen oral liquior was apparently able to downregulate the level of IL-4 secreted by T lymphocytes.This down-regulation was more remarkable when the liquor was given earlier.Conclusion:baoshen oral liquior can markedly promote the activation and proliferation of IgAN murine sphenic B lymphocytes,Increase the functional humoral immunity;Down-regulate the level of IL-4 secreted by T lymphocytes,this appears to be associated with the reduce IgA sedimentation in mesangial areas of glomeriuli.

关 键 词：保肾口服液 IGA肾病 小鼠 B细胞增殖 T细胞分泌 IL-4 

分 类 号：R285[医药卫生—中药学]