金属硫蛋白对海马CA_1区锥体细胞凋亡的抑制作用  

Inhibitory Effect of Metallothionein on the Apoptosis of Pyramidal Cells in CA_1 Region of the Hippocampus

作　　者：刘学忠[1] 崔旭[2] 卞建春 任建新[1] 刘宗平[1] 王捍东[1] 王宗元[1] 

机构地区：[1]扬州大学畜牧兽医学院,江苏扬州225009 [2]解放军总医院老年医学研究所,北京100853

出　　处：《中国兽医学报》2004年第6期581-583,共3页Chinese Journal of Veterinary Science

基　　金：国家重点基础研究发展规划项目 ( G2 0 0 0 0 5 70 0 0 ) ;国家自然科学基金资助项目 ( 3 9870 5 67) ;扬州大学科研基金项目 ( 3 5 5 0 )

摘　　要：采用股动脉放血并双侧颈总动脉夹闭制作全脑缺血再灌注模型 ,于术后 1d处死动物 ,取脑 ,制作石腊切片 ,通过 HE染色、Tunel检测 ,对海马 CA1 区锥体细胞形态进行了观察。结果表明 :在短暂性全脑缺血中 ,海马锥体细胞存在着凋亡和坏死 2种死亡形式 ;与对照组相比 ,缺血再灌注后 1d,海马 CA1 区损伤较重 ,出现了较多的凋亡细胞 ,而MT- 1可明显抑制锥体细胞的凋亡。本试验证明 :在脑缺血再灌注损伤中 ,细胞凋亡是神经元死亡的一种重要形式 ;MT-Bilateral carotid arteries occlusion model of old rats were killed on 1 d after reperfusion following 10 min forebrain ischemia,then their forebrain paraffin sections were made to observe the changes of pyramidal cells in CA_1 region of the hippocampus through HE and Tunel staining,the results showed:apoptosis and necrosis are two patterns of cell death in the course of rat transient global ischemia;the injury of CA_1 region in the hippocampus was severe,lots of apoptotic cell were seen and MT-1 revealed an inhibit effect on the apoptosis of pyramidal cells;the result indicated that apoptosis may be an important form in the course of cerebral ischemia-reperfusion(IR) injury and MT-1 can protect brain tissue from IR injury.

关 键 词：金属硫蛋白 海马 CA1区 锥体细胞 细胞凋亡 抑制作用 

分 类 号：S852.2[农业科学—基础兽医学]