Dynamics of Primary Events in the Photocycle of Excited Bacteriorhodopsin  

作　　者：Jun-Jun LU Ming MING Yi YANG Jia WU Bo LI Jian-Dong DING Qing-Guo LI Shi-Xiong QIAN 

机构地区：[1]Department of Physics,Fudan University,Shanghai 200433,China [2]Department of Macromolecular Science,Key Laboratory of Molecular Engineering of Polymers,Fudan University,Shanghai 200433,China [3]Department of Physiology and Biophysics,School of Life Sciences,Fudan University,Shanghai 200433,China

出　　处：《Acta Biochimica et Biophysica Sinica》2004年第11期724-728,共5页生物化学与生物物理学报（英文版）

基　　金：supported by the grants from the National Natural Science Foundation of China(No.10274103,No.20174006,No.20221402,and No.20374015)

摘　　要：Transient dynamic behavior of the excited bacteriorhodopsin (BR), which was isolated from the strain H. salinarum, was studied at excitation wavelength from 585 to 639 nm. With the one-color femtosecond (fs) pump-probe technique, we revealed the primary events in BR's photocycle that took place in an ultrafast time scale. From the analysis of the decay components of the dynamical traces, it was evident that the isomerization of the retinal chromophore in BR and the intermediate J's formation occurred within 500 fs. BR exhibited pH-dependent dynamical behaviors. When the medium pH was between 5 and 9, the BR ultrafast dynamics has no obvious change. In contrast, the dynamical curves were obviously affected when the pH was out of that region.Transient dynamic behavior of the excited bacteriorhodopsin (BR), which was isolated from the strain H. salinarum, was studied at excitation wavelength from 585 to 639 nm. With the one-color femtosecond (fs) pump-probe technique, we revealed the primary events in BR's photocycle that took place in an ultrafast time scale. From the analysis of the decay components of the dynamical traces, it was evident that the isomerization of the retinal chromophore in BR and the intermediate J's formation occurred within 500 fs. BR exhibited pH-dependent dynamical behaviors. When the medium pH was between 5 and 9, the BR ultrafast dynamics has no obvious change. In contrast, the dynamical curves were obviously affected when the pH was out of that region.

关 键 词：BACTERIORHODOPSIN transient dynamics primary events femtosecond laser pump-probe technique 

分 类 号：Q932[生物学—微生物学]