Accumulation of Pathogenesis-related Type-5 Like Proteins in Phytoplasma-infected Garland Chrysanthemum Chrysanthemum coronarium  

作　　者：Bo-XiongZHONG Yan-WeiSHEN 

机构地区：[1]DepartmentofBioresourceScience,CollegeofAnimalScience,ZhejiangUniversity,Hangzhou310029,China

出　　处：《Acta Biochimica et Biophysica Sinica》2004年第11期773-779,共7页生物化学与生物物理学报（英文版）

摘　　要：Soluble proteins extracted from leaves, apical shoots, axillary shoots, and stems of garland chrysanthemum plants infected by onion yellows phytoplasma were analyzed by two-dimensional gel electrophoresis. Computerized matching analysis revealed that at least six soluble proteins were accumulated specifically in phytoplasma-infected garland chrysanthemum. N-terminal amino acids sequences of these soluble proteins, determined by Edman degradation, shared high sequence similarities with those of patho-genesis-related type-5 (PR-5) proteins such as tobacco thaumatin-like protein. Accumulation of these six proteins was also found in garland chrysanthemum plants infected by other phytoplasmas. These results demonstrate that phytoplasmal infection induces the accumulation of PR-5 like proteins in garland chrysanthemum plants.Soluble proteins extracted from leaves, apical shoots, axillary shoots, and stems of garland chrysanthemum plants infected by onion yellows phytoplasma were analyzed by two-dimensional gel electrophoresis. Computerized matching analysis revealed that at least six soluble proteins were accumulated specifically in phytoplasma-infected garland chrysanthemum. N-terminal amino acids sequences of these soluble proteins, determined by Edman degradation, shared high sequence similarities with those of patho-genesis-related type-5 (PR-5) proteins such as tobacco thaumatin-like protein. Accumulation of these six proteins was also found in garland chrysanthemum plants infected by other phytoplasmas. These results demonstrate that phytoplasmal infection induces the accumulation of PR-5 like proteins in garland chrysanthemum plants.

关 键 词：PHYTOPLASMA garland chrysanthemum Chrysanthemum coronarium pathogenesis-related proteins THAUMATIN OSMOTIN 

分 类 号：Q949.783.5[生物学—植物学] Q51