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作 者:杨庆利[1] 王建飞[1] 丁俊杰[1] 张红生[1]
机构地区:[1]南京农业大学作物遗传与种质创新国家重点实验室,江苏南京210095
出 处:《南京农业大学学报》2004年第4期6-10,共5页Journal of Nanjing Agricultural University
基 金:国家转基因研究和产业化开发专项 (0 3JY10 0 1);江苏省资源开发局专项资助 (KT2 0 0 10 17)
摘 要:采用完全双列杂交设计研究了 7个耐盐性不同的水稻品种苗期耐盐性遗传机制。在水培条件下 ,将亲本和F1世代的 3~ 4叶期幼苗用 8g·L-1的NaCl胁迫处理 10d后 ,测定根系Na+ /K+ 和盐害级别 ,利用主位点组加性显性模型 ,采用向前逐步回归方法对 2个耐盐指标进行遗传分析。结果表明 ,根系Na+ /K+ 的遗传变异符合 2个主位点组 +微位点组的遗传控制模型 ,盐害级别的遗传变异符合 3个主位点组 +微位点组的遗传控制模型。在决定水稻根系Na+ /K+ 和盐害级别的遗传变异中 ,主位点组的加性效应和显性效应共同起作用。The seedlings at 34 leaf stage of a set of diallel crosses,developed from seven rice cultivars with various salt tolerance,were grown in culture solutions containing 8 g·L -1 NaCl for 10 days.It was recorded that the Na+/K+ in root and salinity tolerance rating(STR)of the plants treated by the salt stress to analysis the inheritance of salt-tolerance in rice.A genetic model of major loci group for dominant-additive effects was used to decompose the genotype of parents and crosses into QTL groups.The results showed the inheritance of the Na+/K+ in root was controlled by two major loci groups and minor loci groups,while the salinity tolerance rating was controlled by three major loci groups and minor loci groups.The genetic variation of the Na+/K+ in root and salinity tolerance rating were explained by both additive and dominant gene effects.
关 键 词:水稻 耐盐性 遗传 双列杂交 主位点组加性—显性模型
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