绯寒樱的组织培养快繁研究  

Tissue Culture and Rapid Propagation of Prunus campanulata Maxim.

作　　者：周丽华[1] 王振师[1] 许冲勇[2] 曾雷[1] 龚峥[1] 

机构地区：[1]广东省林科院,广州510520 [2]华南农业大学林学院

出　　处：《广东林业科技》2004年第4期1-4,共4页Forestry Science and Technology of Guangdong Province

基　　金：广东省科技计划项目 (99M 0 3 710G)研究的部分内容之一。

摘　　要：研究了以绯寒樱 (PrunuscampanulataMaxim .)的茎段作为外植体诱导丛生芽发生以及植株再生的培养程序 ,通过正交试验确定了适宜绯寒樱快繁育苗的培养条件 :(1)初代培养基为 1/ 2MS + 6 BA 0 .5mg/L +蔗糖 30 g/L ;(2 )丛生芽诱导培养基为LE + 6 BA 1.5～ 2 .0mg/L +KT 1.5mg/L +蔗糖 30g/L ;(3)生根培养基为 1/ 2MS +IBA 0 .5mg/L +蔗糖 15 g/L。This paper mainly dealt with the study on shoot organog en esis culture in vitro and plantlet regeneration from sprout explants of Prunus campanulata Maxim.. The optimal culture conditions were established by orthog onal test as follows: (1) Initial medium: 1/2MS+6-BA 0.5 mg/L+30 g/L sucrose; (2) Clump bud induction medium: LE+6-BA 1.5～2.0 mg/L+KT 1.5 mg/L+30 g/L suc rose; (3) Rooting medium: 1/2MS+IBA 0.5 mg/L+15 g/L sucrose.

关 键 词：绯寒樱 组织培养 快繁技术 茎段 培养基 

分 类 号：S792.99[农业科学—林木遗传育种]