2,6-二-O-戊基-β-环糊精键合硅胶固定相的合成与色谱性能考察  

Preparation and Evaluation of 2,6-di-O-Pentyl-β-Cyclodextrin Bonded Silica Stationary Phase for High Performance Liquid Chromatography

作　　者：刘立文[1] 罗爱芹[2] 戴荣继[2] 葛晓霞[1] 杨少宁[2] 

机构地区：[1]中国人民武装警察部队学院,河北廊坊065000 [2]北京理工大学,北京100081

出　　处：《色谱》2004年第6期630-633,共4页Chinese Journal of Chromatography

摘　　要：合成了2,6 二 O 戊基 β 环糊精键合硅胶固定相(PCDS)。利用傅里叶红外光谱、X射线光电子能谱、莫氏(Molisch)试验颜色反应对其进行了表征。以邻苯二甲酸二甲酯、甲苯和菲为测试溶质,评价该键合固定相的色谱性能,并考察了该固定相对一些位置异构体的色谱分离效果,讨论了可能的分离机理。In order to improve the chiral separation capability of the conventional β-cyclodextrin bonded-silica gel stationary phase, 2,6-di-O-pentyl-β-cyclodextrin bonded stationary phase(PCDS) was prepared via a long spacer. The resulted bonded-silica stationary phase was characterized by three methods, namely Fourier transform infrared, Molisch color reaction, X-ray optical electrical energy spectrogram. The chromatographic performances of PCDS were investigated by using liquid chromatography with toluene, dimethyl phthalate, and phenanthrene as solutes, and their retention mechanism was investigated and discussed. The results show that the introduction of pentyl to β-cyclodextrin leads to enhancement of the retention of the solutes. The chiral separation capability of the new bonded-silica stationary phase was evaluated by using liquid chromatography with some chiral drugs. Some of the enantiomers such as chlorphenamine maleate and bupropion hydrochloride were separated by heptakis ((2,6-di-O-pentyl))-β-cyclodextrin bonded silica stationary.

关 键 词：高效液相色谱 2 6-二-O-戊基-β-环糊精 键合硅胶 固定相 手性分离 

分 类 号：O658[理学—分析化学]