腹腔镜检查对慢性乙肝HBV DNA含量的影响  

Effect of laparoscopy on HBV DNA of patients with Chronic hepatitis B

作　　者：周丁华 朱武晖 卫冰 王正元 赵玮 黎介寿[2] 

机构地区：[1]解放军二炮总医院普通外科,北京100088 [2]南京军区总医院全军普通外科研究所,江苏南京210002

出　　处：《中国内镜杂志》2004年第11期22-24,共3页China Journal of Endoscopy

基　　金：国家自然科学基金资助项目(批准号:30270406)

摘　　要：目的探讨腹腔镜检查对慢性乙肝HBV DNA含量的影响.方法采用荧光定量PCR检测技术,分析1998年10月～2003年10月间42例慢性乙肝病人行腹腔镜检查后HBV DNA含量的变化.同时,利用自动生化分析仪同步测定其血清中谷丙转氨酶(ALT)和总胆红素(TB)值.结果慢性乙肝病人实施腹腔镜检查后第3天和第7天血清HBV DNA拷贝数、ALT和TB值均分别较术前显著增加(P＜0.01),而术后15 d其血清HBV DNA拷贝数、ALT和TB值与术前相比差异无显著性.结论慢性乙肝病人行腹腔镜检查,可影响乙肝病毒的复制水平,有必要加强围手术期乙肝病毒量及肝功能的监测.Objective: To elucidate the effect of laparoscopy on hepatitis B virus (HBV) DNA of Chronic hepatitis B. Methods: The changes of HBV DNA were analyzed by fluorescent PCR in 42 patients with chronic hepatitis B. Meanwhile, the alanine aminotransferase (ALT) and total bile (TB )were detected by autobiochemics analyzer. Results: The results showed that the serum HBV DNA, ALT, TB were increased remarkably at 3 and 7 days postoperative compared with that at preoperative respectively(P<0.01). Moreover, compared with preoperative, the difference of the serum HBV DNA,ALT,TB showed no statistical significance respectively after 15 days postoperative. Conclusion: To laparoscopy would effect on duplication of hepatitis B virus in patients with chronic hepatitis B. It is necessary to survey the virus quantity and liver function for the perioperation patients with hepatitis B virus.

关 键 词：腹腔镜 慢性乙肝 病毒量 免疫荧光定量PCR方法 

分 类 号：R512.62[医药卫生—内科学]