血红蛋白模拟辣根过氧化物酶在酶联免疫分析中的应用  

Application of Hb as the Mimetic Labeling Enzymeof HRP in the Immunoassay

作　　者：李得加[1] 邹国林[1] 

机构地区：[1]武汉大学生命科学学院,湖北武汉430072

出　　处：《武汉大学学报（理学版）》2003年第6期765-768,共4页Journal of Wuhan University:Natural Science Edition

基　　金：国家自然科学基金资助项目(39770200)

摘　　要：提出了以血红蛋白(Hb)作为辣根过氧化物酶模拟酶,用碳二亚胺法将其标记人IgG,Hb标记人IgG在形成免疫复合物后,仍可保持其95%的催化活性.以邻苯二胺作为氢供体,以羊抗人IgG为分析模型,建立了以血红蛋白作为酶标记物的竞争型免疫分析新体系.人IgG在15～1000μg/L范围内与体系吸光度呈良好的线性关系,线性方程为A=0.297-1.286E-4[IgG](μg/L),相关系数r=0.9992(n=8),检测限为15μg/L.用于人正常血清中人IgG含量的测定,加标回收率为94%～108%,结果令人满意.A carbodiimide method was used to label the human IgG using Hb as the mimetic enzyme of HRP. The immuo-conjugate of Hb with human IgG remained 95% of the catalytic activity of Hb. A new competing type immunoanalytical system was estabilished using Hb as the labeling enzyme, o-phenylenediamine as the hydrogen donor, and the goat anti-human IgG as the analytical model. The concentration of human IgG had a good linear relationship with the absorbance of the system in the concentration range of 15～1 000 μg/L. The linear regression equation A=0.297-1.286E-4\(μg/L), The correlation coefficient was 0.999 2(n=8)and the determination limit was 15 μg/L. The method was used for determination of IgG in normal human serum with good satisfactory results. The analytical recoveries are 94%～108%.

关 键 词：血红蛋白 酶联免疫分析 碳二亚胺 辣根过氧化物酶 模拟 

分 类 号：Q554.6[生物学—生物化学]