Spike protein homology between the SARS-associated virus and murine hepatitis virus implies existence of a putative receptor-binding region  

作　　者：LU Yun  CHEN Yinghua 

机构地区：[1]Laboratory of Immunology, Department of Biology,Protein Science Laboratory of Ministry of Education Tsinghua University, Beijing 100084, China

出　　处：《Chinese Science Bulletin》2003年第11期1115-1117,共3页

基　　金：This work was supported by the Anti-SARS Fund of Ministry of Educaton and the National Natural Science Foundation for Outstanding Young Scientist of China(Grant No.30025038).

摘　　要：Coronavirus has been determined to be the cause of the recent outbreak of severe acute respiratory syndrome (SARS). Human coronavirus 229E had been studied well and its receptor-binding domain was restricted to aa417—547 of S protein. However, this region has no homology with the newly separated SARS-associated virus (Hong Kong isolate CUHK-W1). Then we analyzed the phylogenesis of S1 subunit of the coronavirus spike protein (SARS-associated virus, Hong Kong isolate CUHK-W1). Interestingly, the highest homology between murine hepatitis virus (MHV) and SARS-associated coronavirus was found. And the important sites (aa62—65 and aa214—216) on the spike protein of MHV with receptor-binding capacity were highly conservative in comparison with the newly separated SARS-asso- ciated virus (the corresponding sites are aa51—54 and aa195—197). These results from bioinformatics analysis might help us to study the receptor-binding sites of SARS-associ- ated virus and the mechanism of the virus entry into the target cell, and design antiviral drugs and potent vaccines.Coronavirus has been determined to be the cause of the recent outbreak of severe acute respiratory syndrome (SARS). Human coronavirus 229E had been studied well and its receptor-binding domain was restricted to aa417—547 of S protein. However, this region has no homology with the newly separated SARS-associated virus (Hong Kong isolate CUHK-W1). Then we analyzed the phylogenesis of S1 subunit of the coronavirus spike protein (SARS-associated virus, Hong Kong isolate CUHK-W1). Interestingly, the highest homology between murine hepatitis virus (MHV) and SARS-associated coronavirus was found. And the important sites (aa62—65 and aa214—216) on the spike protein of MHV with receptor-binding capacity were highly conservative in comparison with the newly separated SARS-asso- ciated virus (the corresponding sites are aa51—54 and aa195—197). These results from bioinformatics analysis might help us to study the receptor-binding sites of SARS-associ- ated virus and the mechanism of the virus entry into the target cell, and design antiviral drugs and potent vaccines.

关 键 词：非典型性肺炎冠状病毒 鼠类肝炎病毒 SARS 蛋白质 抗过滤性病源体药物 疫苗 异体同形 

分 类 号：R373[医药卫生—病原生物学]