Secretion and regulatory mechanism of leptin during pregnancy in the golden hamster (Mesocricetus auratus)  

作　　者：WANG Cheng YANG Liguo W. Gen K. Taya 

机构地区：[1]College of Animal Science and Technology, Nanjing Agricultural University,College of Animal Science and Technology, Nanjing Agricultural University,Laboratory of Veterinary Physiology, Tokyo University of Agriculture and Technology,Laboratory of Veterinary Physiology, Tokyo University of Agriculture and Technology Nanjing 210095, China,College of Life Sciences, Xiamen University, Xiamen 361005, China,Nanjing 210095, China,Tokyo 183-8509, Japan,Tokyo 183-8509, Japan

出　　处：《Chinese Science Bulletin》2003年第11期1118-1123,共6页

基　　金：This work was supported by the National Natural Science Foundation of China(Grant No.30070555);short-term exchange program of science and technology(Japan).

摘　　要：The aim of this study is to investigate the secretion pattern and regulatory mechanism of leptin during pregnancy in the golden hamster (Mesocricetus auratus). 100 pregnant golden hamsters were separated into 16 groups and the serum was sampled daily throughout gestation for the leptin assay. Results indicated that a gestational leptin profile existed during pregnancy in the golden hamster. To investigate if endocrine conditions during pregnancy contribute to this gestational leptin profile, serums collected during different reproductive stages were added to the adipocyte culture medium. Results showed that sera collected from all reproductive stages have significant stimulating effect on the secretion of leptin as compared with the control (P < 0.05). Steroid extracted and non-extracted serums from day 2 of estrus cycle (cycling serum ) and day 2 of the lactation have the same effect on the secretion of leptin (P > 0.05). However, sera from mid and late pregnancy significantly stimulate leptin secretion as compared with the cycling sera (P < 0.01). Serum collected on day 15 of pregnancy have the strongest stimulating effect on leptin secretion compared with sera collected on day 8 (P < 0.0001) and day 12 (P < 0.001) of the pregnancy. But after the extraction of steroid hormones, sera collected during different reproductive stages have the same effect on leptin secretion in vitro. Further study showed that dexamethason, progesterone and insulin stimulated while estradiol suppressed leptin secretion in vitro. Our results suggested that steroid hormones (or other steroid factors) have significant regulating effect on the leptin secretion during pregnancy and they may be the most important contributors of the gestational leptin profile in the golden hamster.The aim of this study is to investigate the secretion pattern and regulatory mechanism of leptin during pregnancy in the golden hamster (Mesocricetus auratus). 100 pregnant golden hamsters were separated into 16 groups and the serum was sampled daily throughout gestation for the leptin assay. Results indicated that a gestational leptin profile existed during pregnancy in the golden hamster. To investigate if endocrine conditions during pregnancy contribute to this gestational leptin profile, serums collected during different reproductive stages were added to the adipocyte culture medium. Results showed that sera collected from all reproductive stages have significant stimulating effect on the secretion of leptin as compared with the control (P < 0.05). Steroid extracted and non-extracted serums from day 2 of estrus cycle (cycling serum ) and day 2 of the lactation have the same effect on the secretion of leptin (P > 0.05). However, sera from mid and late pregnancy significantly stimulate leptin secretion as compared with the cycling sera (P < 0.01). Serum collected on day 15 of pregnancy have the strongest stimulating effect on leptin secretion compared with sera collected on day 8 (P < 0.0001) and day 12 (P < 0.001) of the pregnancy. But after the extraction of steroid hormones, sera collected during different reproductive stages have the same effect on leptin secretion in vitro. Further study showed that dexamethason, progesterone and insulin stimulated while estradiol suppressed leptin secretion in vitro. Our results suggested that steroid hormones (or other steroid factors) have significant regulating effect on the leptin secretion during pregnancy and they may be the most important contributors of the gestational leptin profile in the golden hamster.

关 键 词：血清 金色大颊鼠 荷尔蒙 怀孕期 类固醇 激素 分泌物 

分 类 号：Q492.5[生物学—生理学]