Detection of Trichomonas Vaginalis Infection in Male Non-gonococcal Urethritis Patients by InPouch TV Culture and Polymerase Chain Reaction  

作　　者：姚志远 郑和义 曹经江 

机构地区：[1]Department of Dermatology, China-Japan Friendship Hospital [2]Department of Dermatology, Peking Union Medical College Hospital

出　　处：《Chinese Journal of Sexually Transmitted Infections》2002年第3期48-51,共4页中华性传播感染杂志（英文版）

摘　　要：Objective: To study the prevalence of Trichomonas vaginalis (TV) infection in Chinese male patients with nongonococcalurethritis (NGU), to evaluate the sensitivity and specificity ofurine-based and urethral swab polymerase chain reaction(PCR) detection, to set up a method for non-invasive detectionof male TV infection. Method: One hundred and five male NGU patients wereselected from a Beijing STD clinic. Two urethral swabs wereobtained from each patient, one for the InPouch TV culturesystem and the other for PCR. In addition, one first void urinespecimen was collected for PCR detection. Culture wasconsidered the 'gold standard'. The sensitivity, specificity,positive predictive value (PPV) and negative predictive value(NPV) of the two PCR detections were compared to cultureresults. Results: The prevalence of urine-based PCR and urethralswab PCR detection was 3.81% (4/105) and 4.76% (5/105)respectively. Compared to culture, the sensitivity, specificity,PPV and NPV were 80%, 100%, 100% and 99% for urinbased PCR and 80%, 99%, 80% and 99% for urethral swab PCR. Conclusion: TV is one of the etiological agents in male NGU,with a 4.76% prevalence of infection in our study. The urine-based PCR detection has higher sensitivity and specificity andprovides a noninvasive method more feasible in practice.Objective: To study the prevalence of Trichomonas vaginalis(TV) infection in Chinese male patients with nongonococcal urethritis (NGU), to evaluate the sensitivity and specificity of urine-based and urethral swab polymerase chain reaction(PCR) detection, to set up a method for non-invasive detection of male TV infection. Method: One hundred and five male NGU patients were selected from a Beijing STD clinic. Two urethral swabs were obtained from each patient, one for the InPouch TV culturesystem and the other for PCR. In addition, one first void urinespecimen was collected for PCR detection. Culture wasconsidered the “gold standard”. The sensitivity, specificity,positive predictive value (PPV) and negative predictive value(NPV) of the two PCR detections were compared to cultureresults. Results: The prevalence of urine-based PCR and urethralswab PCR detection was 3.81% (4/105) and 4.76% (5/105)respectively. Compared to culture, the sensitivity, specificity,PPV and NPV were 80%, 100%, 100% and 99% for urine-based PCR and 80%, 99%, 80% and 99% for urethral swabPCR. Conclusion: TV is one of the etiological agents in male NGU,with a 4.76% prevalence of infection in our study. The urinebased PCR detection has higher sensitivity and specificity and provides a noninvasive method more feasible in practice.

关 键 词：cell culture polymerase chain reaction URINE trichomonas vaginalis non-gonococcal urethritis 

分 类 号：R696[医药卫生—泌尿科学] R382.21[医药卫生—外科学]