卡那霉素对大豆生长的抑制及筛选试验研究  

STUDY ON CRITICAL AND SCREEN SOYBEAN GROW OF KANAMYCIN

作　　者：袁鹰[1] 刘德璞[1] 王玉民[1] 郑培和[1] 

机构地区：[1]吉林省农业科学院生物技术中心,公主岭136100

出　　处：《大豆科学》2003年第4期261-263,共3页Soybean Science

基　　金：国家"8 63"研究项目子专项"用基因工程技术改良大豆的抗虫性"(2 0 0 1-2 0 0 4)

摘　　要：用 3个大豆品种作为研究材料 ,研究了卡那霉素对种子萌发、子叶节培养、茎尖和组培苗生根的抑制效应。结果表明不同的外植体对卡那霉素的反应存在较大的差异 ,用卡那霉素浸泡种子 ,浓度最好是 10 0 0ppm ,而种子在含卡那霉素培养基上萌发 ,一般为 10 0 - 2 0 0ppm效果较好 ,子叶节在生长培养时卡那霉素可采用 5 0 - 10 0ppm。而茎尖、子叶节组培苗 ,一旦放入含有卡那霉素培养基内 ,不论浓度多少 ,无一生根。不同的基因型亦稍有差异。所以在筛选转化子之前一定要进行临界浓度的筛选试验。The effects of kanamycin on seed germination, growth of cotyledon, rooting of shoots were investigated using 3 soybean varieties The results showed that the effect of kanamycin on different explants varied significantly The critieal concentration was 1000ppm.When soybeans seeds were soaked with kanamycin solution where as the cribical concentration was 50-100ppm,when soybeans were inoculated on media added kanamycin.For cotyledon node ,the certain concentration of kanamycin was 100ppm.None of shoot tips and shoots regenerated from cotyledon cuture could root even in the media with lowest concentration of kanamycin .The effect of kanamycin showed slight on different genotypes.The screening for critical concentration of kanamycin must be done before the screening of transformants.

关 键 词：卡那霉素 大豆 种子萌发 子叶节培养 茎尖 组培苗生根 培养基 转基因植物 

分 类 号：S565.1[农业科学—作物学] Q78[生物学—分子生物学]