洋桔梗叶片培养不定芽发生和微繁研究  

STUDY ON ADVENTITIOUS SHOOT REGENERATION AND MICRO - PROPAGATION FROM LEAVES OF LISIANTHUS (EUSTOMA GRANDIFLORUM)

作　　者：达克东[1] 张松[1] 臧运祥[1] 张志国[2] 吴禄平[3] 

机构地区：[1]山东农业大学园艺学院,山东泰安271018 [2]山东农业大学资源与环境学院,山东泰安271018 [3]沈阳农业大学园艺学院,辽宁沈阳110161

出　　处：《山东农业大学学报（自然科学版）》2003年第4期494-498,共5页Journal of Shandong Agricultural University：Natural Science Edition

摘　　要：以洋桔梗叶片为材料,建立了洋桔梗叶片高效不定芽发生、植株再生体系。研究了不同激素及其组合对外植体不定芽发生、壮苗和生根的效应。结果显示,MS+BA 1mg/L是叶片不定芽发生的适宜培养基;MS+BA 0.1mg/L+NAA 0.05mg/L是试管嫩梢形成壮苗的适宜培养基;MS+IAA 0.1mg/L培养基是壮苗生根的适宜培养基。草炭珍珠岩基质是生根苗移栽的适宜基质。High frequency adventitious shoot formation and plant - let regeneration system were established in leaf culture of lisianthus (Eustoma Grandiflorum) . Effect of plant growth regulators and its combination on adventitious shoot regeneration, plant - let growth and plant - let rooting were studied. The results showed that MS basal medium supplemented with BA 1mg/L was suitable for leaf adventitious shoot formation, MS + BA 0. 1mg/L + NAA 0.05mg/L was suitable for plant - let growth, and MS + IAA 0. 1mg/Lmedium was suitable for plant - let rooting. 1/2 turfysoil + 1/2 vermiculite substrate was suitable for plant - let conversion in greenhouse.

关 键 词：洋桔梗 叶片 激素 不定芽发生 植株再生体系 培养基 微繁研究 

分 类 号：S681.9[农业科学—观赏园艺]