高效液相色谱法测定骨筋丸胶囊中芍药苷含量  

Content of Paeoniflorin in Jinguwan capsule by HPLC

作　　者：黄海萍[1] 何芝义[1] 李瑞莲[1] 

机构地区：[1]湖南省药品检验所,长沙410001

出　　处：《中南药学》2003年第3期154-156,共3页Central South Pharmacy

摘　　要：目的 采用高效液相色谱法测定骨筋丸胶囊中有效成分芍药苷含量。方法 色谱条件为Kromasiil-C18柱,以甲醇-水(25:75)为流动相;检测波长:230 nm;流速1 mL·min-1。结果 芍药苷在0.46-2.30μg范围内具有良好的线性关系,其回归方程为:A=41641.14C-2600.66,r=O.9996。平均回收率为98.6％,RSD=2.48％。结论 本方法操作简单,重现性好,准确,可以用来控制本制剂质量。OBJECTIVE To determine the content of Paeoniflorin in Jinguwan Capsule, by HPLC. METHODS The chromatographic condition was Kromasil-C18 column, the mobile phase was methanol-water (25 : 75); the flow rate was 1 mL · min-1 ; the detection wavelength was 230 nm. RESULTS The standard paeoniflorin showed a good linear correlation within the concentration of 0. 46-2. 30·g, The regress equation was A = 41641. 14C-2600. 66, r = 0.9996; the average recovery was 98. 6%, RSD=2. 48. CONCLUSIONS The method is accurate and simple.

关 键 词：骨筋丸胶囊 芍药苷 含量测定 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]