水蛭素对凝血酶诱导的血管平滑肌细胞增殖的影响  

Influence of Hirudin on Thrombin-induced Proliferation of Vascular Smooth Muscle Cells

作　　者：王敏[1] 崔连群[1] 张承俊[2] 王晓军[1] 烟玉琴[3] 秦风菊[3] 

机构地区：[1]山东大学临床医学院省立医院,山东省济南市250021 [2]济南市第一人民医院,山东省济南市250012 [3]山东科技大学生物医学系,山东省济南市250031

出　　处：《中国动脉硬化杂志》2003年第7期609-612,共4页Chinese Journal of Arteriosclerosis

摘　　要：为研究重组水蛭素对凝血酶诱导的兔胸主动脉血管平滑肌细胞增殖的影响及其作用机制。选用第2～5代培养的血管平滑肌细胞,在与凝血酶(4.0 ku/L)共同孵育的条件下,分别给予水蛭素(6.0 ku/L)和肝素(6.0ku/L)进行干预。通过四唑盐比色实验检测细胞生长活性,流式细胞术检测细胞周期,免疫组织化学检测血小板生长因子和增殖细胞核抗原在血管平滑肌细胞中的表达。结果发现,重组水蛭素能明显抑制凝血酶诱导的平滑肌细胞增殖,其抑制作用可能与减低了血管平滑肌细胞对血小板生长因子和增殖细胞核抗原的表达有关。Aim To study the influence of hirudin on thrombin-induced proliferation of vascular smooth muscle cells (VSMC) and its regulated mechanism in vitro. Methods VSMCs were isolated from the thoracic aorta of rabbits and cultured 2 - 5 passages were incubated with thrombin (4.0 ku/L), while hirudin (6.0 ku/L) and heparin (6.0 ku/L) were added except for control group. The effect of hirudin on growth of VSMC was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltet-razohum bromide (MTT) assay and flow cytometry. The expression of platelet-drived growth factor (PDGF) and proliferating cell nuclear antigen (PCNA) in VSMCs was evaluated by immunohistochemistry technique and computer image analysis system. Results Hirudin remarkedly inhibited both the thrombin-induced proliferation and the expression of PDGF and PCNA in VSMC (P < 0.05, P < 0.01). Conclusion Hirudin is able to significantly influence the proliferative activity of VSMCs, which may be carried out through inhibition of PDGF and PCNA expression.

关 键 词：药理学 水蛭素对血管平滑肌细胞的作用 免疫组织化学染色与流式细胞术 凝血酶 水蛭素 血管平滑肌细胞 动脉粥样硬化 

分 类 号：R96[医药卫生—药理学]