血浆氧化型低密度脂蛋白水平与低密度脂蛋白循环免疫复合物的相关性  

Analyzing the Relationship between in Vivo Oxidized Low Density Lipoprotein and Low Density Lipoprotein Immune Complexes

作　　者：胡兵[1] 汪俊军[2] 张春妮[2] 蔡辉[1] 

机构地区：[1]中国人民解放军南京军区南京总医院中医科,江苏省南京市210002 [2]中国人民解放军南京军区南京总医院全军医学检验中心,江苏省南京市210002

出　　处：《中国动脉硬化杂志》2003年第7期639-642,共4页Chinese Journal of Arteriosclerosis

摘　　要：为探讨血浆氧化型低密度脂蛋白水平与低密度脂蛋白循环免疫复合物间关系。分别建立了以抗人IgG为包被抗体、酶标抗载脂蛋白B为检测抗体的低密度脂蛋白循环免疫复合物酶联免疫吸附试验;以抗氧化型低密度脂蛋白为包被抗体、酶标抗载脂蛋白B为检测抗体的氧化型低密度脂蛋白酶联免疫吸附试验。同时测定60例冠心病患者及50例对照人群低密度脂蛋白循环免疫复合物、氧化型低密度脂蛋白水平并进行相关性分析。结果发现,冠心病患者甘油三酯、脂蛋白(a)和载脂蛋白B水平均显著升高;高密度脂蛋白胆固醇和载脂蛋白AI水平降低;且低密度脂蛋白循环免疫复合物(2.74±0.73比1.38±0.78,P<0.001)、氧化型低密度脂蛋白水平(595.5±194.8比440.3±175.0μg/L,P<0.001)均显著升高。低密度脂蛋白循环免疫复合物水平分别同血浆总胆固醇、甘油三酯、低密度脂蛋白胆固醇、脂蛋白(a)及载脂蛋白B正相关;同载脂蛋白AⅠ负相关。氧化型低密度脂蛋白水平亦分别同血浆总胆固醇、低密度脂蛋白胆固醇、脂蛋白(a)及载脂蛋白B正相关。氧化型低密度脂蛋白水平与低密度脂蛋白循环免疫复合物亦呈正相关(r=0.313,p<0.005)。结果提示,氧化型低密度脂蛋白及循环免疫复合物升高是动脉粥样硬化发生的危险因素。Aim To investigate the possible relationship between oxidized low density lipoprotein (ox-LDL) and LDL-immune complexes (IC) level. Methods We developed a 'sandwich' ELISA for measuring plasma LDL-IC level using anti-human IgG (Fab) as the capture antibody and quantitating with monoclonalanti-apoB enzyme conjugate. Oxidixed LDL was also detected by a sandwich ELISA method using monoclonal antibodies against ox-LDL as the capture antibody and quantitating with anti-apoB enzyme conjugate. Their levels were studied in 60 patients with coronary heart disease (CHD) and 50 control subjects. Results Plasma concentrations of TG, lipoprotein (a) and apolipoprotein B in CHD patients were all significantly increased whereas HDLC and apolipoprotein AI levels were decreased. Plasma LDL-IC (2.74 ± 0.73 AU vs 1.38 ± 0.78 AU, P <0.001) and ox-LDL (595.5 ±194.8 μg/L vs 440.3 ± 175.0 μg/L, P<0.001) concentrations in the patients with CHD were both significantly higher than those of control. The relationships between LDL-IC ox-LDL levels and other lipid traits in all the studied subjects ( n = 110) were carried out. LDL-IC levels were positively correlated with TC, TG, LDLC, lipoprotein(a) and apolipoprotein B concentrations while negatively correlated with apolipoprotein AI concentrations respectively. Similarly ox-LDL levels were also found positively correlated with TC, LDLC, or apolipoprotein B concentrations respectively. Furthermore a significantly positive relation between ox-LDL and LDL-IC levels was found (r = 0.313, P < 0.005). Conclusions Increased oxidized LDL and LDL immune complexes levels are risk factors for atherosclerosis.

关 键 词：生物化学 氧化修饰低密度脂蛋白与循环免疫复合物的关系 酶联免疫吸附测定 冠状动脉疾病 脂蛋白 低密度 氧化型 循环免疫复合物 动脉粥样硬化 

分 类 号：Q513[生物学—生物化学]