对金龟子幼虫有杀虫活性的苏云金杆菌HBF-1菌株发酵培养基优选  

Optimization of Medium for Bacillus thuringiensis HBF-1 with Insecticidal Activity to Scarabaeoidae Larva

作　　者：王容燕[1] 冯书亮[1] 范秀华[1] 曹伟平[1] 胡明峻[1] 

机构地区：[1]河北省农林科学院植物保护研究所,河北保定071000

出　　处：《华北农学报》2003年第F09期96-98,共3页Acta Agriculturae Boreali-Sinica

基　　金：河北省科技攻关项目(01220164D)

摘　　要：通过正交试验优选出对金龟子幼虫有杀虫活性的苏云金杆菌HBF 1菌株的发酵培养基组合为:氮源B4 4%,淀粉1 2%,酵母粉0.5%,KH2PO40.04%,MgSO40.05%。用此配方进行摇瓶发酵,发酵液的平均菌数可达到44亿/mL,晶体产生量为2 1130mg/mL,发酵液稀释50倍对铜绿丽金龟幼虫进行生测,14d平均死亡率达到78 3%。Through orthogone experiment, the optimal medium was obtained for Bacillus thuringinesis HBF-1 with insecticidal activity to scarabaeoidae larva. The medium contained 4.4% nitrogen B, 1.2% starch, 0.5% yeast power, 0.04% KH_2PO_4, 0.05%MgSO_4.Fermentation was done with this medium in shaking flask.Results showed that the number of cells was 44×10~8 cfu/ mL, and the produce of crystal was 2.1130 mg/ mL. The mortality of Anomala corpulenta larva treated with 50-fold of HBF-1 fermented liquid was 78.3% on the 14th day of treatment.

关 键 词：苏云金杆菌 微生物杀虫剂 培养基 金龟科幼虫 杀虫活性 HBF-1菌株 

分 类 号：S433.5[农业科学—农业昆虫与害虫防治] S476.1[农业科学—植物保护]