水稻悬浮细胞系的建立  

The establishment of cell suspension culture of rice

作　　者：袁力勇[1] 李绍清[2] 李阳生[2] 冯双华[2] 戴长柏[1] 李达模[2] 

机构地区：[1]中国医学科学院协和医科大学医学生物学研究所,云南昆明650118 [2]中国科学院中国科技大学长沙农业现代化研究所,湖南长沙410125

出　　处：《云南大学学报（自然科学版）》2003年第4期373-376,共4页Journal of Yunnan University(Natural Sciences Edition)

摘　　要：对包括广亲和材料、恢复系、花培后代在内的30个水稻不同基因型的品种(系)在MS培养基中的培养能力进行了调查.根据出愈率的高低和愈伤组织培养能力,把它们分成Ⅰ,Ⅱ,Ⅲ3种类型.从Ⅰ类型中挑选CPSLO17和IR21820-154-3-2-2-8用N6液体培养基进行液体培养,CPSLO17建立了悬浮细胞系,证实基因型的差异是决定建立良好悬浮细胞系的关键因素之一.此外还考察了低锌胁迫对水稻悬浮细胞系和幼胚愈伤分化率的影响.Immature and mature embryos of 30 rice varieties (lines) were selected and cultured on MS solid medium.According to the callus induction ratio and their response to subculture,they were classified into three groups.CPSLO17 and IR21820-3-2-2-8 showed high callus induction ratio and good response to subculture,were selected to establish the cell suspension culture with N6 liquid medium.The results showed that the genotype is one of the key factors to establish the cell suspensions.With zinc deficiency,the P,Zn contents in suspension cell lines were lower,while P/Zn,Fe/Zn values higher,which is just as the change of mineral elements in rice plant under zinc deficiency.On the other hand,there was little effect on the callus induction frequency,but the differentation ratio decreased under zinc deficiency.

关 键 词：水稻 悬浮细胞系 恢复系 广亲和材料 花培后代 培养基 培养能力 出愈率 液体培养 

分 类 号：S511[农业科学—作物学] Q943.1[生物学—植物学]