银屑病患者皮损中血管内皮生长因子受体的表达与血管增生的关系  

Expression of Vascular Endothelial Growth Factor Receptor in the Lesions of Patients with Psoriasis

作　　者：朱凡[1] 郑敏[1] 鲍彰[1] 

机构地区：[1]浙江大学医学院附属第二医院皮肤科,杭州310009

出　　处：《中华皮肤科杂志》2003年第7期365-367,共3页Chinese Journal of Dermatology

基　　金：浙江省自然科学基金(399126)

摘　　要：目的探讨血管内皮生长因子受体(FLT-1与KDR)在银屑病皮损中的表达及意义。方法采用免疫组化法检测银屑病患者皮损中FLT-1与KDR的表达与分布特征,同时检测皮损中的微血管密度(MVD)。结果在表皮组织中,FLT-1与KDR在患者组与正常人对照组中均有表达,且表达强度差异无显著性(P>0.05);正常人对照组中,FLT-1、KDR均匀表达于表皮全层,而患者组FLT-1、KDR在颗粒层表达最强。真皮组织中,FLT-1、KDR主要表达在真皮乳头微血管上,且患者组真皮乳头微血管上的表达程度要明显高于正常人对照组(P<0.01)。患者组皮损真皮乳头血管MVD明显高于正常人对照组(P<0.01)。真皮乳头微血管FLT-1、KDR阳性表达组的MVD明显大于阴性表达组的MVD(P<0.01)。FLT-1、KDR与MVD三者之间任何两者均存在正相关关系(P<0.01)。结论FLT-1、KDR在表皮角质形成细胞上有表达,其分布在银屑病皮损中发生了变化。真皮乳头微血管上FLT-1、KDR表达的异常增高与银屑病皮损部真皮乳头血管生成有关。这两种受体在血管生成的过程中可能起作用。FLT-1与KDR之间可能也存在相互促进关系。Objective To study the expression of vascular endothelial growth factor(VEGF)recep-tor(FLT-1and KDR)in the lesional skin of psoriasis.Methods Expression of the receptors was examined by immunohistochemical method in tissue sections from28psoriatic patients.The endothelial cells were im-munostained by anti-CD34monoclonal antibody to count the microvessel density(MVD).Results There was no significant difference of the total level of the receptors expressed between the epidermis of psoriasis and the normal controls(P>0.05).These receptors were distributed evenly in all layers of epidermis in nor-mal controls,while intensively in granular layer of lesional skin.The significant overexpression of the recep-tors mainly presented in the papillary dermal microvessels of lesional skin compared to that of normal con-trols(P<0.01).The MVD in lesional skin was higher than that in normal controls(P<0.01).The MVDs were18.36±3.98and19.07±3.71in tissue sections positive for KDR and FLT-1,respectively,while14.25±2.95and13.68±2.81in tissue sections negative for KDR and FLT-1,respectively(P<0.01).The significant positive correlation was found among FLT-1,KDR and MVD.Conclusions VEGF receptors are expressed on the epidermis,but their distribution is significantly different between normal and lesional skin.The up-regulation of FLT-1and KDR on the papillary dermal microvessels of lesional skin may play an important role in the angiogenesis of psoriasis.There may be interaction between these receptors.

关 键 词：FLT-1 KDR 表达 患者 真皮 对照组 正常人 表皮 微血管 血管增生 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]