Epstein-Barr病毒感染与系统性红斑狼疮的相关性研究  

The Association Between Epstein-Barr Virus and Systemic Lupus Erythematosus(SLE)

作　　者：李慧[1] 罗兵[2] 梁华[2] 张海平[1] 孙迎娟[1] 

机构地区：[1]青岛大学医学院附属医院检验科,266003 [2]青岛大学医学院微生物教研室

出　　处：《中华皮肤科杂志》2003年第7期375-377,共3页Chinese Journal of Dermatology

摘　　要：目的探讨Epstein-Barr病毒(EBV)感染在系统性红斑狼疮(SLE)病因学中的作用以及EBV活动性感染与疾病活动期的关系。方法PCR技术检测85例SLE患者和43例正常人外周血单一核细胞中EBVDNA,采用巢式PCR(Nested-PCR)技术检测EBVDNA阳性标本EBV增殖期基因BZLF1mRNA的表达,ELISA法检测血清EBV特异性IgM抗体。结果SLE患者组EBVDNA阳性率明显高于对照组(P<0.05),EBV即刻早期基因BZLF1mRNA的表达在SLE患者组与对照组中阳性率差异无显著性(P>0.05),ELISA法检测血清EBV特异性IgM抗体,SLE患者组阳性率高于对照组(P<0.05)。结论SLE患者EBVDNA阳性率高于正常对照组,部分SLE患者存在EBV活动性感染,EBV感染可能与SLE的发生发展有关。Objective To investigate the possible role of Epstein-Barr virus(EBV)infection in the pathogenesis of systemic lupus erythematosus(SLE)and the association between EBV infection and disease activity in patients with SLE.Methods EBV DNA was detected with PCR from peripheral blood monocytes in85patients with SLE and43age-and sex-matched normal controls.The expression of EBV lytic gene BZLF1was examined by nested PCR in EBV DNA positive samples.Sera EBV-IgM antibody was tested by ELISA.Results The rates of positive EBV DNA were significantly higher in patients than those in controls.There was no significant difference of the expression of lytic gene BZLF1mRNA in EBV DNA positive sam-ples between patients and controls(P>0.05).EBV-specific IgM was detected more frequently in patients than that in controls(P<0.05).Conclusion EBV infection is identified in patients with SLE.It is sug-gested that EBV infection might be related to the pathogenesis of SLE.

关 键 词：EBV SLE 患者 对照组 阳性率 EPSTEIN-BARR病毒 系统性红斑狼疮 DNA RNA PCR) 

分 类 号：R593.241[医药卫生—内科学] R512[医药卫生—临床医学]