自发性高血压大鼠血管平滑肌及心肌内成纤维细胞生长特性的研究  

Study on growth Characteristics of Aortic Smooth Muscle Cell and Cardiac Fibroblast in Spontaneously Hypertensive Rats

作　　者：许昌声[1,2] 陈天宝[1,2] 苏津自[1,2] 林从容[1,2] 谢良地[1,2] 徐尚华[1,2] 

机构地区：[1]福建医科大学附属第一医院高血压研究所 [2]南平市第一医院心内科

出　　处：《中国实验动物学报》1999年第1期31-36,共6页Acta Laboratorium Animalis Scientia Sinica

摘　　要：目的探讨自发性高血压大鼠（ｓｐｏｎｔａｎｅｏｕｓｌｙｈｙｐｅｒｔｅｎｓｉｖｅｒａｔｓ，ＳＨＲ）主动脉平滑肌细胞（ａｏｒｔａｓｍｏｏｔｈｍｕｓｃｌｅｃｅｌ，ＡＳＭＣ）与心肌内成纤维细胞（ｃａｒｄｉａｃｆｉｂｒｏｂｌａｓｔ，ＣＦＢ）在体外培养生长的特性。方法１６周龄自发性高血压大鼠（ＳＨＲ）和正常大鼠（ＷＫＹ）测血压后处死，取心脏与胸主动脉，组织块法分别培养ＣＦＢ与ＡＳＭＣ）。分别观察：（１）ＣＦＢ３Ｈ－ｐｒｏｌｉｎｅ掺入与倍增时间（ＤＴ），ＡＳＭＣ３Ｈ－ＴｄＲ掺入与倍增时间（ＤＴ），比较ＳＨＲ，ＷＫＹ的平滑肌细胞和心肌内成纤维细胞的增殖能力。（２）ＡｎｇｉｏｔｅｎｓｉｎⅡ在无血清或２％小牛血清（ＦＣＳ）条件下对两种大鼠ＡＳＭＣ３Ｈ－ＴｄＲ，ＣＦＢ３Ｈ－ｐｒｏｌｉｎｅ掺入率及细胞数的影响，用３Ｈ－ＴｄＲ，３Ｈ－ｐｒｏｌｉｎｅ分别反应ＡＳＭＣ和ＣＦＢ增殖能力。结果（１）ＳＨＲ的ＡＳＭＣ的３Ｈ－ＴｄＲ掺入率ＣＦＢ３Ｈ－ｐｒｏｌｉｎｅ掺入率显著高于ＷＫＹ，ＡＳＭＣ倍增时间明显短于ＷＫＹ（分别为４７３±０２５，３００±１４０，Ｐ＜００１），而ＣＦＢ两组大鼠间倍增时间无差别（２６１±１６，２６６±１９，?Aim To investigate the growth characteristics of culture aortic smooth muscle cell (ASMC) and cardiac fibroblast (CFB) in spontaneously hypertensive rats (SHR). Method 16 weeks old SHR and WKY were killed after blood pressure measurement, the tissues from heart and aorta were cultured respectively. The following items were observed (1) 3H-Proline incorporation and doubling timing (DT) in CFB; 3H-TdR incorporation and DT in ASMC. (2) The effects of angiotensin Ⅱ(AⅡ) on proliferation of ASMC and CFB from SHR and WKY in the serum free medium or 2% FCS medium. Result (1) 3H-TdR incorporation of SHR ASMC and 3H-proline incorporation of SHR CFB are obviously higher than that of WKY. The DT of SHR ASMC is obviously shorter than that of WKY (30.0±1.40 vs 47.3 ±0.25, P<0.01), while the DT of CFB show no difference between SHR and WKY (26.1±1.6 vs 26.6±1.9, P>0.05). (2) 3H-TdR incorporation of SHR and WKY ASMC was raised to 3.3 and 2.2 times of that of control respectively by 10 -6 mol/L AⅡ(P<0.01); 3H-proline incorporation of SHR and WKY CFB was raised to 3.02 and 2.3 times of that of control respectively by 10 -6 mol/L AⅡ(P<0.01). 3H-TdR incorporation of SHR ASMC and 3H-proline incorporation of SHR CFB stimulated by different concentration AⅡ were much higher than those of WKY(P<0.01), and the effect is corresponded with the AⅡ concentration. AⅡ can also increase the cell number of SHR ASMC and SHR CFB. The incorporation of SHR and WKY can also be promoted while the cell number remains unaltered in serum free medium. Conclusion (1) SHR ASMC and CFB have stronger proliferative ability compared with WKY; (2) AⅡ can enhance the incorporation of ASMC and CFB. AⅡ exhibits a hypertrophic effect in serum free medium and a proliferative effect in low

关 键 词：SHR 自发性高血压大鼠 成纤维细胞 ^3H-TDR掺入 心肌 倍增时间 增殖能力 培养基 生长特性 浓度 

分 类 号：Q813.11[生物学—生物工程] R544[医药卫生—心血管疾病]