Novel Rearrangement of Small Peptides in Electrospray Ionization Tandem Mass Spectrometry  

作　　者：陈益 王津 刘晓红 孙命 陈晶 江洋 赵玉芬 

机构地区：[1]Key Laboratory of Chemical Biology, Department of Chemistry, Zhengzhou University, Zhengzhou, Henan 450052, China [2]Key Laboratory for Bioorganic Phosphorus Chemistry, Ministry of Education, Department of Chemistry, School of Life Sciences and Engineering, Tsinghua University, Beijing 100084, China [3]Department of Chemistry, Peking University, Beijing 100871, China

出　　处：《Chinese Journal of Chemistry》2004年第5期477-481,共5页中国化学（英文版）

基　　金：Project supported by the Chinese National Natural Science Foundation (No. 20175026); the Ministry of Science and Technology; the visiting scholar fund of the Chinese Education Ministry; and Tsinghua University.

摘　　要：Electrospray ionization mass spectrometry (ESI-MS) is a powerful method for sequencing peptides. A novel fragmentation pattern with the loss of a neutral fragment of 45 Da was observed with the dipeptides, tripeptides, tetrapeptides and pentapeptides containing phenylalanine or histidine residues. A novel rearrangement reaction with the extrusion of a formamide piece was studied and the rearrangement mechanism was proposed and confirmed by deuterium labeling experiments with ESI-MSn and high-resolution mass spectrometry. These findings are poten-tially helpful in identifying the specific sequence pattern in the peptide sequencing.Electrospray ionization mass spectrometry (ESI-MS) is a powerful method for sequencing peptides. A novel fragmentation pattern with the loss of a neutral fragment of 45 Da was observed with the dipeptides, tripeptides, tetrapeptides and pentapeptides containing phenylalanine or histidine residues. A novel rearrangement reaction with the extrusion of a formamide piece was studied and the rearrangement mechanism was proposed and confirmed by deuterium labeling experiments with ESI-MSn and high-resolution mass spectrometry. These findings are poten-tially helpful in identifying the specific sequence pattern in the peptide sequencing.

关 键 词：REARRANGEMENT small peptides electrospray ionization tandem mass spectrometry 

分 类 号：O657.7[理学—分析化学]