Enzymatic Synthesis of a CCK-8 Tetrapeptide Fragment  

作　　者：GuangYaXIANG HeinerECKSTEIN 

机构地区：[1]PharmacySchoolofTongjiMedicalCollege,HUST,Wuhan430030 [2]InstituteofOrganicChemistry,UniversityofTiibingen,Tiibingen,D-72076,Germany

出　　处：《Chinese Chemical Letters》2004年第7期768-770,共3页中国化学快报（英文版）

摘　　要：The enzymatic synthesis of a tetrapeptide Phac-Met-Gly-Trp-Met-OEt is reported. It was synthesized by coupling Phac-Met-OEt with Gly-OMe ·HC1, Trp-OMe and Met-OEt successively, catalyzed by α-chymotrypsin, papain and α-chymotrypsin respectively. The results of FAB-MS showed that the products had the correct molecular mass.The enzymatic synthesis of a tetrapeptide Phac-Met-Gly-Trp-Met-OEt is reported. It was synthesized by coupling Phac-Met-OEt with Gly-OMe ·HC1, Trp-OMe and Met-OEt successively, catalyzed by α-chymotrypsin, papain and α-chymotrypsin respectively. The results of FAB-MS showed that the products had the correct molecular mass.

关 键 词：CCK-8 enzymatic method Α-CHYMOTRYPSIN papain. 

分 类 号：Q516[生物学—生物化学]