Interaction of Haematopoietic Tissue Cultures of the Dublin Bay Prawn,Nephrops Norvegicus(L.),with the Causal Agent of Luminous Vibriosis Vibrio Harveyi  

作　　者：Mulford A.L. ZHANG X.H. XU H.S. Austin B. 

机构地区：[1]Department of Biological Sciences,Heriot-Watt University,Riccarton,Edinburgh,EH144AS,Scotland,UK [2]Department of Marine Biology,Ocean University of Qingdao,Qingdao 266003,P.R.China

出　　处：《Journal of Ocean University of Qingdao》2002年第1期66-70,共5页青岛海洋大学学报(英文版)

基　　金：The support of European Union grant(FAIR-CI97-3413)is gratefully acknowledged.

摘　　要：Vibrio harveyi cells(dose>103 cells mL-1)and extracellular products(ECP;>25μg mL-1 of total protein concentration)destroyed haematopoietic cultures of Nephrops norvegicus within 24 h of exposure.Cytopathic effects(CPE)started after 4 h of exposure to the bacterial cells,with some granularity in the cytoplasm,mostly in cells in the outer periphe-ry of the explant growth.At the end of the infection,a considerable number of nuclei remained attached to the substrate,apparently unaffected.Following exposure to ECP,initial deterioration was observed at 2 h with the presence of granularity in the cytoplasm of<20%cells,and few cells displayed small vacuoles around the nuclei.Parallel results were obtained using whole animal experiments,with V.harveyi cells being lethal to nephrops within 24 h.

关 键 词：Vibrio haryeyi Dublin Bay prawn haematopoietic tissue INTERACTION 

分 类 号：Q25[生物学—细胞生物学]