冬凌草愈伤组织诱导及细胞培养的研究  

Studies on the Induction of Calli and Cell Culture of Rab dosia rubescens

作　　者：李景原[1] 王太霞[1] 杨相甫[1] 张晋豫[1] 李贺敏[2] 

机构地区：[1]河南师范大学生命科学学院,新乡453002 [2]河南农业大学农学系

出　　处：《中草药》2000年第12期938-941,共4页Chinese Traditional and Herbal Drugs

摘　　要：用组织培养、细胞悬浮培养和单细胞平板培养技术，诱导出冬 凌草愈伤组织，并探讨 了细胞悬浮培养时间、培养方法和接种密度对冬凌草单细胞平板培养植板率的影响。结果表 明：从冬凌草叶和嫩茎诱导愈伤组织，以 MS+2，4D 1 mg/L+NAA 0.5 mg/L 培养基较好 ，愈 伤组织诱导率高达 96.80%。用普通单细胞平板培养法培养冬凌草单细胞的植板率很低，而 以悬浮培养 15～18 d 的单细胞为材料，接种密度为 5×103个/毫升时，进行条件培养 和 看护培养，植板率达 21.63%。本研究结果可供利用细胞培养技术筛选冬凌草高产冬 凌草素细胞株时参考。Conditions for cell culture of Rabdosia rubes cens (Hemsl) Hara. were studied with the aim to develop a new source of ru bescensin for antitumor therapy. Calli were induced from the leaf and stem of R. rubes cens and cultured either by cell suspension culture or unicellular plate c ulture. Fac tors affecting plate efficiency, such as cell suspension culture time, ways of p la ting and cell density were studied. Culture medium MS containing 1∶0.5 ratio of 2, 4D∶NAA was found to be the most suitable medium for the induction of calli which attained an induction rate well over 96.8%. High pla ting efficiency could be obtained by plating at a density of 5×103 cells/mL w ith monocells separated from 15.18 d cells.Result of the present study may pro vide some reference for the screening of high yield cell strain for the producti on of rubescensin.

关 键 词：冬凌草 愈伤组织 细胞培养 中医药疗法 组织培养 细胞悬浮培养 

分 类 号：R269.4[医药卫生—中西医结合]