Selection of Immunogenic Peptide Mimics of Male Worm Origin of Schistosoma Japonicum using Phage Display Peptide Library  

作　　者：陈欲晓 易新元 曾宪芳 王林纤 唐连飞 章洁 Mcreynolds Larry 

机构地区：[1]Department of Immunology, Xiangya School of Medicine, Central South University [2]Department of Parasitology, Xiangya School of Medicine, Central South University [3]Molecular Parasitology Group, New England Biolabs, Inc.

出　　处：《Journal of Microbiology and Immunology》2004年第1期66-69,共4页中华微生物学和免疫学（英文版）

基　　金：ThisprojectwassupportedbyWHO TDR(No.980268)

摘　　要：To select the immunogenic short peptide mimics of male worm origin of Schistosoma japonicum (Sj) and to explore their protection effect against schistosomiasis in mice, the random phage display peptide library of 12-mer was screened with IgG to soluble male worm antigen of Sj, and the specific positive clones selected through three rounds of screenings were detected by Dot-ELISA, and then injected subcutaneously into mice for vaccination and protection assessment against Sj. It was found that 18 randomly picked phage displayed clones all showed definite antigenicity with various intensities. The pooled phages displayed clones could induce production of specific antibodies and cause 31.72% of worm reduction rate and 51.54% of egg reduction rate in mice, revealing a significant difference (P<0.001) in comparison with those of the controls. It concludes that the short peptide mimics of male worm origin of Sj obtained by affinity screening phage display peptide library can elicit partial protection against this pathogen.To select the immunogenic short peptide mimics of male worm origin of Schistosoma japonicum (Sj) and to explore their protection effect against schistosomiasis in mice, the random phage display peptide library of 12-mer was screened with IgG to soluble male worm antigen of Sj, and the specific positive clones selected through three rounds of screenings were detected by Dot-ELISA, and then injected subcutaneously into mice for vaccination and protection assessment against Sj. It was found that 18 randomly picked phage displayed clones all showed definite antigenicity with various intensities. The pooled phages displayed clones could induce production of specific antibodies and cause 31.72% of worm reduction rate and 51.54% of egg reduction rate in mice, revealing a significant difference (P<0.001) in comparison with those of the controls. It concludes that the short peptide mimics of male worm origin of Sj obtained by affinity screening phage display peptide library can elicit partial protection against this pathogen.

关 键 词：Schistosoma japonicum Male worm Short peptide mimic Phage peptide library Screening 

分 类 号：R383.24[医药卫生—医学寄生虫学] R392[医药卫生—基础医学]