Multianalyte Biosensor for Simultaneous Determination of Glucose and Galactose Based on Micromachined Chamber-type Electrodes  

作　　者：贾能勤 章宗穰 朱建中 张国雄 

机构地区：[1]Department of Chemistry, College of Life and Environmental Science,Shanghai Normal University,Shanghai 200234,China [2]State Key Laboratory of Transducer Technology,Shanghai Institute of Metallurgy,Chinese Academy of Sciences,Shanghai 200050,China

出　　处：《Chinese Journal of Chemistry》2004年第9期908-912,共5页中国化学（英文版）

基　　金：Shanghai Rising Star Project of Shanghai Science and Technology Committee (No. 04QMX1443) the Science Foundation of Shanghai Education Commission and Applied Chemistry Key Laboratory Foundation of Shanghai Normal University.

摘　　要：An amperometric multianalyte biosensor for the simultaneous determination of glucose and galactose was de-veloped based on chamber-type electrodes, which were fabricated by micromachining technology. The dual cham-ber-type enzyme electrode with glucose and galactose sensor elements was integrated onto one microchip. The ex-perimental parameters of this biosensor were optimized. The biosensor exhibited a linearity of up to 4.0 mol/L for glucose and 4.5 mol/L for galactose, and the response time was about 30 s for glucose and 40 s for galactose. No cross-talking behavior was investigated in the course of simultaneous measurement of the two analytes. Interference from electroactive species, such as ascorbic acid and uric acid, was minimized due to the permselectivity of Nafion film. In addition, the biosensor displayed a storage stability of longer than one month.An amperometric multianalyte biosensor for the simultaneous determination of glucose and galactose was de-veloped based on chamber-type electrodes, which were fabricated by micromachining technology. The dual cham-ber-type enzyme electrode with glucose and galactose sensor elements was integrated onto one microchip. The ex-perimental parameters of this biosensor were optimized. The biosensor exhibited a linearity of up to 4.0 mol/L for glucose and 4.5 mol/L for galactose, and the response time was about 30 s for glucose and 40 s for galactose. No cross-talking behavior was investigated in the course of simultaneous measurement of the two analytes. Interference from electroactive species, such as ascorbic acid and uric acid, was minimized due to the permselectivity of Nafion film. In addition, the biosensor displayed a storage stability of longer than one month.

关 键 词：multianalyte biosensor micromachining technology chamber-type electrode 

分 类 号：O657.1[理学—分析化学]