PCR法快速识别Actinobacteria的五种模板制备方法的比较  

Comparison of Five PCR Template Preparation Methods for Fast Identification of Actinobacteria

作　　者：张玉琴[1] 李文均[1] 陈国忠[1] 杨颖[1] 田新朋[1] 

机构地区：[1]云南大学省微生物研究所教育部微生物资源开放研究重点实验室,昆明云南650091

出　　处：《生物技术》2004年第5期37-39,共3页Biotechnology

基　　金：国家自然科学基金 (30 2 70 0 0 4 );云南省自然科学基金项目(No .2 0 0 1C0 0 0 1Q);云南省教教育厅基金项目 (No .0 2QJ0 77)

摘　　要：放线细菌的 2 3SrRNA基因序列具有较好的保守性和相对的可变性 ,被认为是快速筛选放线细菌的合适部位。能否快速有效地扩增特异性区间 ,聚合酶链反应 (PCR)扩增前的模板制备质量是关键 ,故比较了基因组DNA作为PCR模板的 5种制备方法。旨在寻找准确、快速、简便、经济的放线细菌筛选技术 ,为普通和极端环境放线细菌资源的研究和开发利用创造条件。The 23S rRNA gene of Actinobacteria is always considered as a suitable location for fast screening Actinobacteria from bacteria because it is conserved and variable.It is critical to get high quality samples before PCR amplification.Therefore five genomic DNA extraction methods were compared with each other.The aim of this study is to search one new method, which could be used as accurately,fast,easily and economically screening Actinobacteria,providing a platform for exploration and utilization Actinobacteria resources under normal and extreme environments.

关 键 词：放线细菌 Chelex-100法 基因组DNA提取 

分 类 号：Q523[生物学—生物化学]