针刺对老年大鼠脾脏细胞因子基因表达的影响  

Effects of Acupuncture on Cytokine Gene Expression in Aged Rat Spleen

作　　者：黄诚[1] 陈汉平[1] 秦秀娣[1] 周羚[1] 翟道荡[1] 程介士[2] 

机构地区：[1]上海市针灸经络研究所,200030 [2]上海医科大学医学神经生物学国家重点实验室,200032

出　　处：《上海中医药大学上海市中医药研究院学报》1996年第2期106-108,共3页

摘　　要：为了进一步探讨针灸抗衰老机制,我们应用逆转录.聚合酶链式反应(RT-PCR)技术观察腹腔注射细菌脂多糖(LPS)后老年大鼠脾脏白细胞介素1β(IL-1β)、白细胞介素/受体拮抗剂(IL-lra)、白细胞介素6(IL-6)mRNA 水平及针刺后的变化。结果表明,老年大鼠脾脏 IL-1β、IL-lra 和 IL-6mRNA 低于青年大鼠,针刺组则有所提高。提示针刺提高老年机体免疫功能可能与促进细胞因子基因表达有关。In order to further clarify the mechanism of acupuncture in anti-aging,the authors observed the expression of IL-1β,IL-lra and IL-6 mRN As in aged rat spleen following introperitoneal injection of lipopolysaccharide(LPS)and the effects of acupunc- ture on those mRNAs by means of the reverse transcription polymerase chain reaction(TR-PCR) technique.The results showed that IL-1β,IL-1ra and IL-6 mRNAs in aged rat spleen were significantly lower when compared with the young rat and acupuncture could increase the expression,sugge- sting that increase of cytokine gene expression might be responsible for the enhancement of immune function in aged rats following acupuncture.

关 键 词：针刺 老年大鼠 脾脏 细胞因子 基因表达 针灸 抗衰老 

分 类 号：R245[医药卫生—针灸推拿学]