毛细管电泳法快速测定枳实和枳壳中的橙皮甙和柚皮甙  

Fast Determination of Hesperidin and Naringin in Fructus Aurantii Immaturus and Fructus Aurantii by Capillary Electrophoresis

作　　者：吕元琦[1,2] 王月伶[3] 袁倬斌[3] 

机构地区：[1]德州学院化学系 [2]中国科学院研究生院应用化学研究所,北京100039 [3]中国科学院研究生院应用化学研究所

出　　处：《生命科学仪器》2004年第5期36-37,共2页Life Science Instruments

基　　金：“十五”科技攻关重大项目(2001BA210A04-7);国家自然科学基金(20175025);国家自然科学基金分析化学重点基金(20235010);电分析化学国家重点实验室基金;“九五”科技攻关重点项目(96-A23-01-06)的资助

摘　　要：A method was developed to quickly determine hesperidin and naringin in Fructus Aurantii Immaturus and Fructus Aurantiiby capillary electrophoresis. Through the investigation of the effects of buffer pH and concentration, applied voltage, organic solvent, SDSconcentration and β-CD, the analytical conditions were optimized. Under the optimized conditions, the two analytes were well separatedin 5 min. A good linear relationship between the peak area and concentration was found in the 6-500 μg/mL and 5-500 μg/mLconcentration range for hesperidin and naringin, respectively. The relative standard deviation based on migration time and peak area were0.82%、1.16% and 2.95%、3.08% for hesperidin and naringin, respectively. The detection limits based on three time noise were 1.2μg/mL and 1.5μg/mL for hesperidin and naringin, respectively. The method was verified by real sample analysis and runningstandard addition and recovery experiments with satisfactory results.A method was developed to quickly determine hesperidin and naringin in Fructus Aurantii Immaturus and Fructus Aurantiiby capillary electrophoresis. Through the investigation of the effects of buffer pH and concentration, applied voltage, organic solvent, SDSconcentration and β-CD, the analytical conditions were optimized. Under the optimized conditions, the two analytes were well separatedin 5 min. A good linear relationship between the peak area and concentration was found in the 6-500 μg/mL and 5-500 μg/mLconcentration range for hesperidin and naringin, respectively. The relative standard deviation based on migration time and peak area were0.82%、1.16% and 2.95%、3.08% for hesperidin and naringin, respectively. The detection limits based on three time noise were 1.2μg/mL and 1.5μg/mL for hesperidin and naringin, respectively. The method was verified by real sample analysis and runningstandard addition and recovery experiments with satisfactory results.

关 键 词：枳实 枳壳 未成熟 柚皮甙 毛细管电泳法 橙皮甙 《神农本草经》 果实 幼果 栽培变种 

分 类 号：R282[医药卫生—中药学] R284[医药卫生—中医学]