β-环糊精修饰区带毛细管电泳法测定野菊花中的刺槐素、木犀草素和槲皮素  

Determination of Acacetin, Luteolin and Quercetin in Chrysanthem indicum L by β-CD modified Capillary Electrophoresis

作　　者：吕元琦[1,2] 王月伶[3] 袁倬斌[3] 

机构地区：[1]德州学院化学系 [2]中国科学院研究生院应用化学研究所,北京100039 [3]中国科学院研究生院应用化学研究所

出　　处：《生命科学仪器》2004年第5期38-39,共2页Life Science Instruments

基　　金：“十五”科技攻关重大项目(2001BA210A04-7);国家自然科学基金(20175025);国家自然科学基金分析化学重点基金(20235010);电分析化学国家重点实验室基金;“九五”科技攻关重点项目(96-A23-01-06)的资助

摘　　要：A method was developed to determine acacetin, luteolin and quercetin in Chrysanthem indicum L by β-CD modifiedcapillary electrophoresis. Through the investigation of the effects of buffer pH and concentration, applied voltage, SDS concentration andβ-CD, the analytical conditions were optimized. Under the optimized conditions, the two analytes were well separated in 8 min. A goodlinear relationship between the peak area and concentration was found in the 2-400μg/mL,2-500 μg/mL and 2-500μg/mLconcentration range for acacetin, luteolin and quercetin, respectively. The relative standard deviation based on migration time and peakarea were 0.51%, 0.76%, 0.83% and 2.17%, 2.42%, 2.68% for acacetin, luteolin and quercetin, respectively. The detection limits basedon three time noise were 0.6μg/mL, 0.5μg/mL and 0.6μg/mL for acacetin, luteolin and quercetin, respectively. The method wasverified by real sample analysis and running standard addition and recovery experiments with satisfactory results.A method was developed to determine acacetin, luteolin and quercetin in Chrysanthem indicum L by β-CD modifiedcapillary electrophoresis. Through the investigation of the effects of buffer pH and concentration, applied voltage, SDS concentration andβ-CD, the analytical conditions were optimized. Under the optimized conditions, the two analytes were well separated in 8 min. A goodlinear relationship between the peak area and concentration was found in the 2-400μg/mL,2-500 μg/mL and 2-500μg/mLconcentration range for acacetin, luteolin and quercetin, respectively. The relative standard deviation based on migration time and peakarea were 0.51%, 0.76%, 0.83% and 2.17%, 2.42%, 2.68% for acacetin, luteolin and quercetin, respectively. The detection limits basedon three time noise were 0.6μg/mL, 0.5μg/mL and 0.6μg/mL for acacetin, luteolin and quercetin, respectively. The method wasverified by real sample analysis and running standard addition and recovery experiments with satisfactory results.

关 键 词：野菊花 毛细管电泳法 槲皮素 木犀草素 黄酮类化合物 疗效 栓剂 刺槐 花序 有效成分 

分 类 号：TH133.3[机械工程—机械制造及自动化] R284[医药卫生—中药学]