重瓣矮牵牛叶片的组织培养  

Study on Tissue Culture of Petunia Hybrida

作　　者：崔广荣[1] 梁继田 崔海[1] 

机构地区：[1]安徽技术师范学院农学系,安徽凤阳233100 [2]安徽省长丰县庄墓职业中学,安徽长丰231151

出　　处：《特产研究》2004年第3期20-23,共4页Special Wild Economic Animal and Plant Research

基　　金：安徽技术师范学院自然科学基金项目(ZRC200212)

摘　　要：以矮牵牛叶片为外植体进行愈伤组织诱导、丛芽直接诱导的研究,同时也进行了芽苗生根试验。研究结果表明:两类植物生长调节剂细胞分裂素和生长素的不同浓度配比对叶片体外培养特性影响较大,直接诱导成苗以MS+6-BA3.2mg/L+NAA0.1mg/L和MS+6-BA1.6mg/L+NAA0.2mg/L较为适宜,其余处理多形成愈伤组织。添加了不同浓度生长素NAA或IBA的生根培养基均表现出一定的对生根的抑制作用,不添加任何激素的1/2MS固体培养基最为适合矮牵牛的生根。The leaves of petunia hybrida were used as explants to research the induction of callus, crowd shoot and rooting.The results indicated that the leaves of petunia hybrida producing crowd shoots or calli in vitro were greatly influenced by the concentration of two kinds of hormones.The appropriate solid culture medium for crowd shoots induction is MS+6-BA 3.2mg/L+NAA 0.1mg/L and MS+6-BA 1.6mg/L+MAA 0.2mg/L, while the proper culture medium for rooting is 1/2 MS without any hormones.

关 键 词：重瓣矮牵牛 叶片 组织培养 外植体 培养基 

分 类 号：S681.6[农业科学—观赏园艺]