两种光敏化合物光诱导稗草细胞产氧自由基电子自旋共振的检测与分析  

Assay of the gross content of oxygen radical in calli of barnyard grass induced by two photosensitive compounds under ultraviolet irradiation by ESR

作　　者：杨淑娟[1] 万树青[1] 蒋志胜[2] 于奡[1] 尚稚珍[3] 

机构地区：[1]华南农业大学资环学院 [2]南开大学元素有机化学所,天津300071 [3]南开大学中心实验室,天津300071

出　　处：《植物保护学报》2004年第3期294-298,共5页Journal of Plant Protection

基　　金：广东省自然科学基金(990703;010319);南开大学元素有机所国家重点实验室资助课题(2002)

摘　　要：采用电子自旋共振光谱仪,检测两种光敏化合物α-三连噻吩(α-T)和1-苯基-4-(3,4-亚甲基二氧)苯基-丁二炔(简称化合物5)处理的稗草愈伤组织后,经近紫外光光照与非光照处理,诱导细胞内产氧自由基的总量。试验结果表明:两种光敏化合物在1mg/L 的浓度下,光照组与非光照组愈伤组织产氧自由基的总量存在显著差异,α-T 处理的光照与非光照电子自旋共振图谱氧自由基积分面积与愈伤组织量的比值分别是54.58和46.78mm^2/mg;化合物5的氧自由基积分面积与愈伤组织量的比值分别是68.62和53.98mm^2/mg。而两种光敏化合物处理的非光照组与空白对照处理的光照组与非光照组所诱导的氧自由基总量不存在显著差异。表明两种光敏化合物对稗草生长的光活化抑制作用与光活化Ⅰ型机制有一定的关系。The technique of ESR was exploited to assay the gross content of oxygen radical inthe calli of barnyard grass,E.crusgalli,induced by two photosensitive compounds,alpha-ter-thienyl(α-T)and 1-phenyl-4-(3,4-methylenedioxy)-phenyl-diacetylene(compound 5),un-der UV-A.The result showed that there was marked difference in the content of oxygen radicalin calli treated by the two compounds under light and absence of light.The rate of integral areaof ESR spectra(mm^2)and mass of calli(mg)of α-T under light and absence of light was54.58 mm^2/mg and 46.78 mm^2/mg,compound 5 was 68.52 mm^2/mg and 53.98mm^2/mg,re-spectively.But there was no marked difference in the content of oxygen radical in calli treatedby the two compounds between absence light and the control,light and absence of light.It willbe seen from this experiment that I-type of photoactivation is major mechanism of inhibition tobarnyard grass,E.crusgalli,under UV-A.

关 键 词：光敏化合物 光诱导 稗草细胞 氧自由基 电子自旋共振 检测分析 

分 类 号：S451.1[农业科学—植物保护]