用于识别不同细胞蛋白质组的噬菌体抗体芯片  

Phage Antibody Chip for Discriminating Proteomes from Different Cells

作　　者：洪龙[1] 廖玮[2] 魏芳[2] 赵新生[2] 朱圣庚[1] 

机构地区：[1]北京大学生命科学学院 [2]分子动态与稳态结构国家重点实验室,北京大学化学与分子工程学院化学生物学系,北京100871

出　　处：《物理化学学报》2004年第10期1182-1185,共4页Acta Physico-Chimica Sinica

基　　金：国家自然科学基金(29973003;20333010);国家重点基础研究发展规划(G1999075305)资助项目~~

摘　　要：将4个鼠源噬菌体抗体克隆和1个人源噬菌体抗体克隆偶联到羧基终止的硅片表面,制成分析型模型芯片.挑选健康人体淋巴细胞为正常细胞的代表,HeLa细胞为肿瘤细胞的代表,提取细胞的全部蛋白质并用荧光染料Cy3标记,与制成的分析芯片反应,得到了不同的结合图谱.实验结果表明,以噬菌体抗体为分子感受器的分析芯片可用于识别不同细胞的蛋白质组.A model analytical chip was prepared by coupling 4 phage antibody clones from mouse and I clone from human to the surface of carboxyl-terminated silicon. The lymphocytes from healthy donors were selected as normal cells, while HeLa cells were selected as tumor cell. The whole proteins were labeled by fluorescent dye Cy3 after isolation from lymphocytes and HeLa cells, and then reacted with the analytical chip. A different binding pattern was obtained. Our results showed that the protein chip made of phage antibodies was able to recognize the proteome from different cells.

关 键 词：细胞 蛋白质组 噬菌体抗体芯片 噬菌体抗体库 生物传感器 

分 类 号：Q819[生物学—生物工程]