新疆家蚕抗菌肽(Cecropin-XJ)特性的研究  

Studies on the Properties of Cecropin-XJ from Xinjiang Silkworm

作　　者：刘忠渊[1,2] 张富春[1,2] 蔡伦[3] 赵干[3] 单文娟[3] 钟哲[3] 王宾[3] 

机构地区：[1]新疆大学生命科学与技术学院分子生物学重点实验室 [2]新疆生物资源基因工程重点实验室,新疆乌鲁木齐830046 [3]新疆生物资源基因工程重点实验室

出　　处：《新疆农业科学》2004年第F08期26-31,共6页Xinjiang Agricultural Sciences

基　　金：新疆自治区高技术研究发展计划项目 ( 2 0 0 3 11118);新疆大学校;院联合资助项目 ( 2 0 0 3 3 2 0 60 2 )的内容

摘　　要：探讨了新疆家蚕抗菌肽 (Cecropin -XJ)的抗菌特性。根据琼脂糖孔穴扩散法 ,检测抗菌肽的热稳定性、抑菌效价、对氨苄青霉素抗性菌的抑菌作用 ,检测抗菌肽对酸碱盐、人造胃液的耐受性及其抗菌谱。结果表明新疆家蚕抗菌肽 (Cecropin -XJ)具有很强的热稳定性、能够杀灭氨苄青霉素抗性菌、对酸碱盐、人造胃酸有一定的耐受性 ,其杀菌活力为 :1μg/ μl抗菌肽与 2 4 0 0 0单位的氨苄青霉素杀菌活力相当。新疆家蚕抗菌肽能够杀灭革兰氏阳性菌和革兰氏阴性菌 ,而对革兰氏阳性菌的抗菌活力高于革兰氏阴性菌 ,这一发现将为抗菌肽在农业、医疗卫生、畜牧业等方面的应用奠定基础 。The purpose of this study is to investigate the properties of recombinant Cecropin-XJ isolated from Xinjing silkworm and expressed in pichia yeast. By Agar Hole Pervasion tests, this recombinant Cecropin-XJ has exhibited an extreme heat-stable characteristic and the ability to kill ampicillin-resistant S. aureus. Moreover, we have observed that the Cecropin-XJ well tolerant to extreme acidic, basic, and high salt environments as well as resistant to 24 hours digestion by artificial gastric juice. The inhibition capability to S. aureus with 1μg/μl Cecropin-XJ is equal to 24 000U ampicillin. With a broad spectrum of antibacterial activities, the Cecropin-XJ is able to inhibit the Gram-positive bacteria and Gram-negative bacteria. However, it has exhibited a better inhibition to Gram-positive bacteria than that of Gram-negative bacteria. These findings could lead it to a broad applications for agriculture, medical, domestic animal and food industry. The further investigation of the antibacterial mechanism of Cecropin-XJ is needed.

关 键 词：新疆家蚕抗菌肽 抗菌特性 琼脂糖孔穴扩散法 热稳定性 抑菌效价 人造胃液 

分 类 号：Q966[生物学—昆虫学]