4种石斑鱼亲缘关系的RAPD分析  

RAPD Analysis of Four Grouper Species

作　　者：郑莲[1] 刘楚吾[1] 

机构地区：[1]湛江海洋大学水产学院,广东湛江524025

出　　处：《水产科学》2004年第11期16-20,共5页Fisheries Science

基　　金：广东省重大科技兴海资助项目(A200099A01)

摘　　要：采用9个引物,对湛江沿海野生蜂巢石斑鱼、鲑点石斑鱼、点带石斑鱼及黑边石斑鱼的基因组DNA进行了RAPD分析,研究结果表明:蜂巢石斑鱼、鲑点石斑鱼、点带石斑鱼及黑边石斑鱼种间遗传距离(Dij)分别为0 4009(蜂巢石斑鱼-鲑点石斑鱼)、0 4805(蜂巢石斑鱼-点带石斑鱼)、0 5703(蜂巢石斑鱼-黑边石斑鱼)、0 4409(鲑点石斑鱼-点带石斑鱼)、0 4561(鲑点石斑鱼-黑边石斑鱼)及0 4729(点带石斑鱼-黑边石斑鱼);用UPGMA法对这4种石斑鱼进行聚类分析可知,蜂巢石斑鱼与鲑点石斑鱼首先聚在一起(0 4009),而后蜂巢石斑鱼-鲑点石斑鱼与点带石斑鱼聚在一起(0 4611),最后,蜂巢石斑鱼-鲑点石斑鱼-点带石斑鱼再与黑边石斑鱼聚在一起(0 4951)。在所研究的类群中,蜂巢石斑鱼和鲑点石斑鱼有较近的亲缘关系。Genotypes of four wild grouper species (Epinephelus merra Bloch, E. fario, E. malabaricus and E. fasciatus) in Zhanjiang littoral are analysed using 9 random primers by the method of random amplified polymorphic DNA (RAPD). The results sugguest that: (1) The inter- species genetic distance(Dij) are 0.4009(E .merra and E. fario), 0.4805 (E. merra and E. malabaricus), 0.5703 (E. merra and E. fasciatus), 0.4409 (E. fario and E. malabaricus), 0.4561(E. fario and E. fasciatus) and 0.4729 (E. malabaricus and E. fasciatus). (2) According to UPGMA, first, E. merra and E. fario are clustered together (0.4009); second, they and E. malabaricus are clustered (0.4611), finaly, all of them are clustered (0.4951). Therefore, the relationship of E. merra and E. fario is closer.

关 键 词：石斑鱼 亲缘关系 RAPD分析 蜂巢石斑鱼 鲑点石斑鱼 点带石斑鱼 

分 类 号：S965.334[农业科学—水产养殖]