菊花叶盘片转基因再生体系的优化选择  

Optimization Selection of Genetic Transformation Regeneration System from Leaves of Dendranthema morifolium

作　　者：刘军[1] 赵兰勇[1] 丰震[1] 张美蓉[1] 吴银凤[2] 

机构地区：[1]山东农业大学林学院,泰安271018 [2]青岛职业技术学院,青岛266071

出　　处：《植物学通报》2004年第5期556-558,共3页Chinese Bulletin of Botany

摘　　要：以菊花(Dendranthema morifolium)无菌苗叶片为外植体,在芽诱导培养基上直接诱导植株再生,优化选择菊花转基因再生体系。筛选出了能直接诱导芽再生的培养基(MS+6-BA3 mg.L-1+NAA 1 mg.L-1)。茎段在有IBA和NAA的1/2MS培养基上诱导生根。生根的小苗在温室里生长良好。A genetic transfomation regeneration system from aseptic seedling leaves of chrysanthe-mum (Dendranthema morifolium) was developed. Adventitious shoots were directly induced andregenerated from leaf explants of chrysanthemum, and the medium (MS+6-BA 3 mg.L-1+NAA 1 mg.L-1), which could directly induce shoot regeneration, was used. Shoots were rooted on half-strengthMS media with IBA and NAA. The rooted plantlets grew well in greenhouse.

关 键 词：菊花 外植体 再生体系 转基因 叶盘 

分 类 号：S682.11[农业科学—观赏园艺]