香榧体细胞胚发生的研究  

Studies on the Somatic Embryogenesis of Torreya grandis

作　　者：姜新兵[1] 陈力耕[1] 何新华[1] 

机构地区：[1]浙江大学园艺系,农业部园艺植物生长发育与生物技术重点开放实验室,杭州310029

出　　处：《园艺学报》2004年第5期654-656,共3页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目 (3 0 0 70 63 4) ;高等学校博士点基金项目 (2 0 0 3 0 3 3 5 118)

摘　　要：以香榧胚为外植体进行体细胞胚的诱导 ,在SH +6 BA 0 1mg/L +2 4 D 0 5mg/L的培养基上愈伤组织诱导率可达到 6 9 8%;愈伤组织经过数次继代后转移至 1 /2SH +6 BA 0 1mg/L +NAA 0 1mg/L培养基上可诱导体细胞胚的形成 ,诱导率可达 70 3%,体细胞胚在合适的培养基上可继续生长和增殖。这是国内外首次通过组织培养成功获得香榧体细胞胚的报道。Calli were induced from embryo of Torreya grandis, and the highest induction frequency(69.8%) was obtained on SH basal medium supplemented with 6-BA 0.1 mg/L+24-D 0.5 mg/L. After subcultured for several times, somatic embryogenesis at high frequency(70.3%) was obtained on 1/2 SH medium supplemented with 6-BA 0.1 mg/L+NAA 0.1 mg/L. Somatic embryos can keep on growing and proliferating on appropriate medium. It is the first reported that somatic embryos were successfully cultured from T. grandis in the world.

关 键 词：香榧 体细胞胚发生 愈伤组织 培养基 

分 类 号：S664.5[农业科学—果树学]