温度对GF_(43)李离体保存中老化作用的影响  

Effects of Temperature on Senescence of GF_(43) (Prunus domestica L.) Plantlet in Vitro

作　　者：马瑞娟[1] 俞明亮[1] 许建兰[1] 宋卫平[2] 洪法水[2] 万志刚[2] 

机构地区：[1]江苏省农业科学院园艺研究所,南京210014 [2]苏州大学生命科学学院,苏州215006

出　　处：《园艺学报》2004年第5期660-662,共3页Acta Horticulturae Sinica

基　　金：江苏省自然科学基金项目 (BK2 0 0 2 5 0 1)

摘　　要：研究了不同温度对GF43 李 (PrunusdomesticaL .)试管苗生长及老化过程的影响。结果表明在 1 6℃保存条件下GF43 试管苗的营养生长缓慢 ,8℃保存GF43 试管苗的营养生长极缓慢 ,SOD、CAT和POD等保护酶系统的活性维持较高水平 ,体内O·2 自由基、丙二醛 (MDA)含量较低 ,细胞膜结构相对稳定 ,从而延缓了试管苗的衰老 ,延长了两次继代之间的时间 ,对试管苗的保存十分有利。The growth and senescence of GF_ 43(Prunus domestica L.)cultured at different temperatures in vitro were studied. The results showed that the growth rate of GF_ 43 plantlet in vitro was slow at 16℃, the activities of superoxide dismutase(SOD), catalase(CAT)and peroxidase(POD)were incresed, the activated oxygen scarenging system was improved. Consequently this alleviated the harmful effect of O·_2 and reduced the membrane lipid peroxidation in shoot, prolonged the life-spane of GF_ 43 plantlet in vitro.

关 键 词：温度 GF43李 离体保存 老化作用 

分 类 号：S662.3[农业科学—果树学]