球根海棠离体培养的形态发生研究  

In Vitro Morphogenesis of Begonia tuberhybrida

作　　者：刘福平[1] 苏明华[1] 章宁林 清洪 

机构地区：[1]福建省亚热带植物研究所,厦门361006

出　　处：《园艺学报》2004年第5期694-696,共3页Acta Horticulturae Sinica

基　　金：福建省重大科技项目 (99-I-1) ;福建省自然科学基金项目 (C0 410 0 40 )

摘　　要：球根海棠外植体可在同一条件下同时诱导不定芽和胚状体 ,经间接途径和直接途径的体细胞胚胎发生频率均很高 ,体细胞胚为单细胞起源或多细胞起源。以再生嫩叶为继代中间体 ,在较高温度(2 8℃ )下诱导 ,可建立高效扩繁体系。纵切芽丛继代形成大量愈伤组织 ,愈伤组织继代半年后几乎完全丧失分化能力 ,发生激素驯化作用 ,而且愈伤组织细胞染色体数目已发生广泛变异。Organogenesis and somatic embryogenesis of Begonia tuberhybrida could carry out on the same culture conditions. Somatic embryos occurred from explant directly or indirectly and originated from single-cell or multi-cell. At higher induce temperature(28℃), subculturing regenerated young leaf could establish high efficient regenerate system. Through subculturing cluster of buds,a large amount of callus formed , and the callus subcultured for half a year lost almostly differential ability. Observation showed callus was habituated by growth substance and there were wide-ranging variation in the number of chromosome in cells of the callus.

关 键 词：球根海棠 离体培养 形态发生 外植体 

分 类 号：S685.99[农业科学—观赏园艺]