黄精多糖对老年糖尿病小鼠脑组织糖基化终产物受体mRNA表达的影响  

Effect of polygona polysaccharose on expression of RGEP mRNA in brain of the aged diabetic rat

作　　者：吴燊荣[1] 李友元[2] 邓洪波[2] 肖洒 王蓉[2] 

机构地区：[1]中南大学湘雅医学院附属海口医院老年病科,海口市570208 [2]中南大学湘雅医学院附二院老年病科 [3]海口市中医药学校

出　　处：《中华老年医学杂志》2004年第11期817-819,共3页Chinese Journal of Geriatrics

基　　金：海南省自然科学基金重点资助项目 (No 30 2 2 3)

摘　　要：目的　探讨黄精多糖对老年糖尿病小鼠脑组织糖基化终产物受体mRNA (RAGEmRNA)表达的调节作用。　方法　BALB/C小鼠 30只 ,随机分为对照组、糖尿病模型组和治疗组 ,每组 1 0只 ;糖尿病模型组和治疗组腹腔注射链脲佐菌素建立小鼠糖尿病模型 ,治疗组给予黄精多糖2ml/kg ,每日 1次灌胃给药 ,共 1 2周。各组实验动物脑组织RAGEmRNA表达的测定采用RT PCR法。　结果　糖尿病模型组实验鼠脑组织RAGEmRNA表达增加 ,RAGE/ β actin相对值 (0 1 5 3±0 0 5 4 )明显高于对照组 (0 ,P <0 0 1 )。应用黄精多糖治疗后 ,治疗组鼠脑组织RAGEmRNA表达较模型组降低 ,RAGE/ β actin相对值 (0 0 92± 0 0 33)显著低于模型组 (P <0 0 5 )。　 结论　黄精多糖能抑制老年糖尿病鼠脑组织RAGEmRNA表达 ,对高血糖及糖基化终产物 (AGE)Objective To study the regulative effect of polygona polysaccharose on the mRNA expression of RGEP(receptor of glycosy lated end products) in brain of aged diabetic rats Methods Diabetic rat model was made by injecting streptozotocin into its abdominal cavity RT PCR method was used to examine the expression of RAGE mRNA in rat brain Results RAGE mRNA in tissue of brain in aged diabetic rats was increased The value of RAGE/ actin (0 153±0 054) in aged diabetic rats was significantly higher than that in the control group ( P <0 01) After the treatment of polygona polysaccharose the expression of RAGE mRNA in tissue of brain was decreased in the model group The RGEP/ actin value in the treated group(0 092±0 033) was significantly lower than that in the animal model group( P <0 05) Conclusions Polygona polysaccharose may decrease the expression of RGEP mRNA in the tissue of brain in aged diabetic rats and has protective effect on the tissue damage caused by hyperglycemia and advanced glycosy lated end products

关 键 词：黄精多糖 老年人 糖尿病 小鼠 脑组织 受体 mRNA表达 RAGE mRNA 糖基化终产物 多糖类 

分 类 号：R587.1[医药卫生—内分泌] R742[医药卫生—内科学]