大鼠甲状旁腺细胞经培养后再移植的实验研究  

Experimental Study on Allotransplantation of Cultured Parathyroid Cells in Rats

作　　者：段秀庆[1] 宋纯[2] 王江滨[1] 石于波[2] 许评[1] 宋春芳[1] 

机构地区：[1]哈尔滨医科大学第一临床医学院普外科,哈尔滨150001 [2]哈尔滨医科大学第一临床医学院卫生部细胞移植重点实验室

出　　处：《中国普外基础与临床杂志》2004年第6期486-488,共3页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的　研究大鼠甲状旁腺细胞经培养后再移植对其存活的影响。方法　将经胶原酶和胰蛋白酶消化的甲状旁腺细胞培养后再行移植 ,观察移植物的存活情况 ,并对移植物做透射电镜观察。结果　新鲜甲状旁腺移植组平均存活期为 (9.2 5± 3.4 5 )d ;甲状旁腺细胞培养后移植 ,移植物的存活时间为 (46 .2 5± 7.4 4 )d ,明显延长(P<0 .0 1) ,在 5 0d观察期内 ,8只鼠中有 6只的血清钙及PTH值持续在正常范围内。移植物内可见完整的甲状旁腺细胞 ,其内见丰富的粗面内质网、杆状的线粒体及分泌颗粒。结论　大鼠甲状旁腺细胞经培养后再移植可以延长移植物的存活时间 ,是治疗甲状旁腺功能低下症的一条有效途径。Objective To study the influence of transplantation of cultured parathyroid cells on the survival of the allografts in rats. Methods Parathyroid cells digested with collagenase and trypsin were cultured and transplanted under the left renal capsule. The survival time of the allografts was recorded and the allografts were examined by transmission electron microscopy.Results In fresh parathyroid cells group, the mean survival time was (9.25± 3.45) days. While in cultured parathyroid cells group, the survival time was (46.25±7.44) days ( P <0.01). During the 50 days of observation, serum calcium and PTH remained normal in 6 of 8 rats. There were intact parathyroid cells in the allografts which had abundant rough endoplasmic reticula,mitochondria and secretory granules. Conclusion Transplantation of cultured parathyroid cells in rats can prolong the survival time of allografts and is a potent way to cure hypoparathyroidism.

关 键 词：大鼠 甲状旁腺移植 细胞培养 实验 

分 类 号：R653[医药卫生—外科学]