丹参注射液及其活性成分丹参素、原儿茶醛对人红细胞胞浆Ca^(2+)浓度影响的实验研究  

Effect of danshensu, protocatechualdehyde and Danshen injection on calciumion concentration in cytoplasm of human erythrocytes

作　　者：沈玲红[1] 王彬尧[1] 王长谦[1] 谢秀兰[1] 俞国瑞 姚中一 朱元龙 杨葆津 

机构地区：[1]上海第二医科大学附属仁济医院心内科,上海200001 [2]上海本草生物医学工程研究所,上海200040

出　　处：《中国中药杂志》2004年第10期984-988,共5页China Journal of Chinese Materia Medica

摘　　要：目的 :探讨丹参水溶性部位钙拮抗作用的主要有效成分。方法 :采用荧光探针Fura 2定量分析法 ,分别测定丹参注射液及其活性成分丹参素、原儿茶醛对离体健康成人红细胞胞浆Ca2 + 浓度的影响。结果 :丹参素和原儿茶醛均有降低成人红细胞胞浆Ca2 + 浓度的作用 ,并表现出剂量依赖性 ,二者合并用药 ,药效相加 ,作用与丹参注射液相似。结论 :丹参素和原儿茶醛可能是丹参注射液钙拮抗作用的主要有效成分。Objective: To study the predominant calcium-an ta gonist components of Danshen injection. Method:The effects of danshensu, protocatechualdehyde and Danshen injection on calcium concentratio n in cytoplasm of erythrocytes were examined in vitro by the fluorescent Ca 2+ -chelator fura-2. Result: Either DS182 or PCAD can d ecrease in dose-dependent cytosolic free calcium concentration in human erythro cytes. They had additive effect when mixed, which was similar to Danshen inje ction. Conclusion: DS182 and PCAD may be predominant calcium- antagonist components of Danshen injection. [

关 键 词：丹参注射液 活性成分 丹参素 原儿茶醛 红细胞 实验研究 钙离子 

分 类 号：R285.5[医药卫生—中药学]