用RAPD技术对人参栽培群体的遗传分析  

Genetic analysis of cultivated ginseng population with the assistance of RAPD technology

作　　者：邵爱娟[1] 李欣[1] 黄璐琦[1] 魏建和[2] 林淑芳[1] 

机构地区：[1]中国中医研究院中药研究所,北京100700 [2]中国医学科学院协和医科大学药用植物研究所,北京100094

出　　处：《中国中药杂志》2004年第11期1033-1036,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金重点项目 (3 993 0 2 2 0 ) ;国经贸厅行业 [2 0 0 3 ] 2 2号

摘　　要：目的 :通过对栽培人参不同品系之间的遗传多样性进行研究 ,为人参品种选育提供参考。方法 :采用SPSS 10 0软件计算Dice遗传相似性系数 ,Between groupslinkage方法聚类 ,构建亲缘关系系统图。结果 :共筛选出18个 10碱基随机引物用于PCR扩增 ,17个供试样品共获得 14 5条扩增片段 ,其中有 5 3条是多态的 ,占 36 5 %。聚类分析结果表明 :大马牙类型内各品系间的亲缘关系近于与二马牙类型各品系样品间的亲缘关系 ,从分子水平证明了根据表征性状对栽培类群进行划分是可行的。结论 :RAPD对人参新品种培育进行辅助选择是一种良好的分子标记。Objective: To provide reference for breeding of c ultivated ginseng by researching the genetic diversities among different strains . Method: To make up the systematic diagram of genetic relatio nship by calculating the proximity coefficient with SPSS 10.0 software and clus tered by between-groups linkage method. Result: The selected 18 random primers used in PCR amplification to produce 145 bands in 17 samples , among which 53 bands (36.5%) are polymorphic. The results of cluster analys is show that the genetic relationship between different strains of Da-maya is c loser than that of Er-maya, which proves that it is feasible to differentiate the cultivated groups in molecular level on outside character. Conclus ion: RAPD is a favorable molecular marker to assist ginseng breeding. [

关 键 词：RAPD技术 人参 栽培 遗传分析 中药 

分 类 号：S567.5[农业科学—中草药栽培]