RP-HPLC同时测定苦黄注射液中4种大黄蒽醌类成分含量  

Simultaneous determination of 4 anthraquinones in Kuhuang injection by RP-HPLC

作　　者：吴永江[1] 陈建军[1] 程翼宇[1] 

机构地区：[1]浙江大学药学院中药科学与工程学系,浙江杭州310027

出　　处：《中国中药杂志》2004年第11期1041-1044,共4页China Journal of Chinese Materia Medica

基　　金：国家重点基础研究发展规划项目 (973计划 )资助(G19990 944 0 5 )

摘　　要：目的 :建立同时测定苦黄注射液中大黄素、大黄酸、大黄酚和大黄素甲醚 4种大黄蒽醌类成分含量的反相高效液相色谱分析方法。方法 :采用汉邦LichrospherC18柱 ,流动相组成为A :1%醋酸水溶液 ,B :含 1%醋酸的80 %乙腈水溶液 ,梯度洗脱 ,流速 0 8mL·min-1,柱温 35℃ ,检测波长 2 5 4nm ,以外标法定量。结果 :苦黄注射液中 4种蒽醌类成分的平均加样回收率分别为大黄酸 98 9%、大黄素 10 0 5 %、大黄酚 10 2 5 %和大黄素甲醚 99 0 % ,线性范围分别为大黄酸 0 0 875～ 1 75 μg ,大黄素 0 0 82 5～ 1 6 5 μg ,大黄酚 0 15 9～ 3 17μg和大黄素甲醚 0 0 5 2 5～ 1 0 5μg。 结论 :本法准确可靠 ,重现性好 ,结果稳定 ,适合于苦黄注射液中这 4种蒽醌含量的分析。Objective: To develop a new high-performance l iq uid chromatographic method for simultaneous quantitative determination of 4 anth raquinones in Kuhuang injection. Method: The chromatographic s eparation was performed on a Lichrospher C 18column (4.6 mm×250 mm,5 μ m,), A linear gradient elution of A (CH 3COOH∶H 2O=1∶100) and B (CH 3COOH ∶H 2O∶CH 3CN=1∶20∶80) was used. The flow rate was 0.8 mL·min -1an d column temperature was set at 35 ℃. The UV detection wavelength was set at 2 54 nm. Result: The recoveries of rhein, emodin, chrysophano l and physcion were 98.9%, 100.5%, 102.5% and 99.0%, respectively. Their linear ranges were 0.087 5～1.75 μg for rhein, 0.082 5～1.65 μg for emod in, 0.159～3.17 μg for chrysophanol and 0.052 5～1.05 μg for physcion, r espectively. Conclusion: The method was accurate, repeatable and suitable to determine the contents of 4 anthraquinones in Kuhuang injectio n. [

关 键 词：反相高效液相色谱法 苦黄注射液 蒽醌类成分 中药 

分 类 号：R286[医药卫生—中药学]