O157大肠杆菌生物学特性及分离鉴定技术研究  

Biological Characteristics of E.coli O157 and Technology of Isolation and Identification

作　　者：顾玲[1] 郭喜玲[1] 李玉青[2] 曾晓燕[1] 史智扬[1] 汪华[1] 

机构地区：[1]江苏省疾病预防控制中心,江苏南京210009 [2]北京市疾病预防控制中心

出　　处：《疾病监测》2004年第10期368-371,共4页Disease Surveillance

摘　　要：目的　研究肠出血性大肠杆菌 (EnterohemorrhagicEscherichiacoli)O15 7:H7和其他O15 7大肠杆菌 (以下简称O15 7:非H7)的生物学特性 ,制定高效、实用的O15 7:H7分离程序及鉴定指标。方法　生化试验和玻片凝集试验分别检测菌株的生化特性和菌体抗原 ;穿刺法接种抗 -H7抗血清琼脂检测H7鞭毛抗原 ,试管凝集试验检测其它鞭毛抗原。聚合酶链反应 (PCR)分别检测特异性基因和毒力基因。结果　 194株O15 7:H7与10 1株O15 7:非H7在山梨醇、蔗糖、赖氨酸和鸟氨酸等生化反应中有差异。 194株O15 7:H7中 192株(98 97% )携带O15 7特异性基因 ,189株 (97 4 2 % )菌体抗原表型阳性 ;10 1株O15 7:非H7均携带O15 7特异性基因同时表型亦为阳性。 194株O15 7:H7均携带H7特异性基因 ,186株 (95 88% )鞭毛抗原表型阳性。 194株O15 7:H7中除 1株 (0 5 2 % )四种毒力基因检测均为阴性外 ,其余 193株 (99 4 8% )均携带 2种及其以上毒力基因 ;10 1株O15 7:非H7四种毒力基因检测均为阴性。结论　O15 7:H7特征性生化反应、O15 7和H7特异性基因检测及其携带毒力基因的特性 ,在区分不同类型的O15 7方面有重要意义 ,据此可以制定针对性强的O15 7:H7分离鉴定程序。Objective To study the biological characteristics of EHEC O157:H7 and other E.coli O157 (hereinafter called as O157: non-H7) so as to establish highly efficient and practical O157:H7 isolation procedure as well as identification index. Methods Biochemical test and slide agglutination test were used respectively to determine the biological characteristics and O antigen of strains; Antiserum agar inoculated with anti-H7 by puncture was used to detect H7 flagellar antigen and tube agglutination test was adopted to determine other flagelar antigen. Polymerase Chain Reaction (PCR) was used to test specific genes and toxicity genes, respectively. Results 194 strains of O157:H7 and 101 strains of O157: non-H7 had differences in biochemical reaction including sorbitol, sucrose, lysine and ornithine, etc. 192 strains of the total 194 (98.97%) carried O157 specific gene and 189 strains (97.42%) had positive antigen phenotype; 101 strains of O157: non-H7 all carried O157 specific gene and the phenotype was positive simultaneously. 194 strains of O157:H7 all carried H7 specific gene and the flagellar antigen of 186 strains (95.88%) was positive. Except one within 194 strains of O157:H7 had negative results of 4 toxicity genes, the other 193 strains all carried 2 or more toxicity genes; the testing of 4 toxicity genes in 101 strains of O157:non-H7 were all negative. Conclusion The characteristic biochemical reaction of O157:H7, determination of O157 and H7 specific genes as well as the feature of toxicity genes had a great importance in differentiating O157 of various types, on the basis of which highly pertinent O157:H7 isolation and identification procedure would be established.

关 键 词：O157 大肠杆菌 生物学特性 分离技术 鉴定技术 聚合酶链反应 

分 类 号：R378.21[医药卫生—病原生物学]