髓复康调节脊髓半横断损伤后自由基变化的实验研究  

Study on Effect of Suifukang in Regulating the Change of Free Radical after Hemitransected Spinal Cord Injury

作　　者：赵永青[1] 田伟[1] 韩凤岳[1] 

机构地区：[1]中国中医研究院骨伤科研究所,北京100700

出　　处：《中国中西医结合杂志》2004年第11期996-998,共3页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然基金资助项目(No.30171189)

摘　　要：目的探讨中药复方髓复康对实验性脊髓损伤后脊髓组织和血液中自由基变化的调节作用。方法70只雄性成年SD大鼠随机分为4组,即髓复康组(20只)、激素组(20只)、对照组(20只)和正常组(10只),前3组建立脊髓T12右侧半横断损伤模型。髓复康组和对照组造模前3天和造模后2h分别灌胃髓复康和等量生理盐水1次,激素组术后立即腹腔注射甲基泼尼松30mg/kg1次,分别于术后8h和24h各取10只大鼠测定血清和脊髓损伤区组织SOD活性和MDA含量。结果(1)SOD活性(血清和脊髓组织)与正常组比较,造模后8h和24h对照组其活性均降低(P<005),髓复康组和激素组大鼠SOD活性变化无统计学意义。(2)MDA含量(血清和脊髓组织)造模后8h,与正常组比较,各损伤组脊髓组织中MDA含量上升(P<005),且对照组明显高于髓复康组和激素组(P<005);24h时髓复康组和激素组比同组8h时明显下降(P<005),对照组8h和24hMDA含量均高于髓复康组和激素组(P<005)。结论髓复康能有效清除脊髓损伤后血清和损伤脊髓组织中过多的自由基和提高机体抗氧化能力。To explore the regulatory effect of Suifukang (SFK), a compound Chinese patent drug on the change of free radical in tissue of spinal cord and blood caused by experimental spinal injury. MethodsSeventy male adult SD rats were randomly divided into 4 groups, the SFK group (n=20), the hormone group (n=20), the control group (n=20) and the normal group (n=10). Rats in the former three groups were made into the right hemitransected spinal cord (T12) injury. SFK were given to the rats in the SFK group 3 days before and 2 hrs after modeling via gastrogavage, saline was given to rats in the control group at the same time. As for the rats in the hormone group, methyl prednisolone 30 mg/kg was given once immediately by peritoneal injection. The rats were sacrificed in two batches (10 of each group in each batch) at 8 hrs and 24 hrs after modeling to obtain the serum and injured spinal cord tissue for determining superoxide dismutase (SOD) activity and malondialdehyde (MDA) content. Results(1) As compared with the normal group, SOD activity in serum and spinal tissue of the control group was lower significantly both at 8 hrs and 24 hrs after modeling, but the changes of SOD activity in the SFK and the hormone group were insignificant; (2) Content of MDA in serum and spinal tissue of rats elevated after modeling, which in the control group at 8 hrs after modeling were higher than that in the normal group, also higher than that in the SFK group and the hormone group; but at 24 hrs after modeling, it lowered significantly in both treated groups. The changes of MDA content in serum were similar to those in the spinal tissue. ConclusionSFK could effectively eliminate the excessive free radical in serum and injured spinal tissue, and raise the capability of antioxidation of organism.

关 键 词：髓复康 脊髓损伤 超氧化物歧化酶 丙二醛 中药 

分 类 号：R285[医药卫生—中药学]