环维黄杨星D对大鼠心律失常双向作用的电生理研究  

Electrophysiologic Study of the Biphasic Effects of Cyclovirobuxine D on Arrhythmias

作　　者：陈章强[1] 胡申江[1] 史维雅[1] 杜娟[1] 沈岳良[2] 夏强[2] 

机构地区：[1]浙江大学医学院附属第一医院心内科,杭州310003 [2]浙江大学医学院生理教研室

出　　处：《中国中西医结合杂志》2004年第11期1010-1013,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家教育部留学回国人员科研启动基金资助(No.1999-747)

摘　　要：目的通过环维黄杨星D(CVBD)对大鼠离体心室乳头肌的电生理作用,探讨其抗心律失常和致心律失常的可能机制。方法采用标准玻璃微电极技术,观察记录大鼠离体右心室乳头肌跨膜电位。结果(1)CVBD在13.3～63.3μmol/L剂量范围内呈剂量依赖性延长动作电位复极化时程(APD),主要延长APD50、APD70和APD90;在33.3～63.3μmol/L剂量范围内,呈剂量依赖性抑制静息电位(RP)、动作电位幅度(APA)和0相最大去极化速率(Vmax)。(2)CVBD还具有时间依赖效应,当20μmol/L灌流心室肌10min后开始出现作用,随着药物作用时间的延长,APD50、APD70和APD90也逐渐增大,但是到30～40min左右时作用达最高峰,此后并不继续延长。(3)CVBD明显延长动作电位的有效不应期(ERP)、增大ERP与APD的比值。(4)CVBD在较高浓度(33.3μmol/L)作用超过45min后有些细胞出现频发、多源性自发性活动。结论CVBD具有抗室性心律失常作用,其机制与延长心室肌细胞动作电位时程及其有效不应期(ERP)和增大ERP与APD的比值有关,同时也具有致心律失常作用,其致心律失常作用可能与抑制RP、APA、Vmax以及过度延长APD有关。To explore the possible mechanism of cyclovirobuxine D (CVB D) in countering and inducing arrhythmia, by way of studying its electro physiological effect on ventricular papillary muscles of rats in vitro. MethodsThe transmembrane potential of rat's isolated right ventricular papillary muscles were recorded using conventional glass micro electrode technique. Results (1) CVB D in concentration of 13 3 63 3 μmol/L, showed prolonging effect on the action potential repolarization time, mainly the action potential duration 50 (APD 50 ) , APD 70 and APD 90 , in dose dependent manner, in concentration of 33 3 63 3 μmol/L, it could inhibit the resting potential, action potential amplitude (APA) and maximum depolarization velocity (Vmax) in dose dependent manner. (2) CVB D also showed time dependent effect, the effect initiated 10 min after 20 μmol/L was perfused in ventricular muscle, the APD 50 , APD 70 and APD 90 were potentiated gradually along with prolongation of action time and reached the peak at 30 40 min, without any potentiation thereafter. (3) CVB D could markedly prolong the effective refractory period (ERP) of action potential, increase the ratio of ERP/APD. (4) CVB D in concentration of 33 3 μmol/L could induce frequent, multifocal spontaneous arrhythmia in some cells when the action time was longer than 45 min. ConclusionCVB D has the action of anti ventricular arrhythmia, the mechanism is correlated with the prolongation of APD and ERP of ventricular muscle as well as the increase of ERP/APD ratio, while it also has the effect of inducing arrhythmia, the mechanism might be concerned with excessive prolongation of APD and the inhibition on RP, APA and Vmax.

关 键 词：环维黄杨星 右心室乳头肌 心律失常 电生理 黄杨宁 

分 类 号：R285[医药卫生—中药学]