黄芪降低糖尿病大鼠心肌黏附分子表达并保护心肌细胞超微结构  

Radix astragali to reduce the expression of adhesion molecule and protect the ultrastructure of myocardium of diabetic rats

作　　者：俞淑静[1] 赵家军[1] 高聆[2] 焦玉莲[2] 周晶[3] 

机构地区：[1]山东大学山东省立医院内分泌科,250021 [2]山东大学山东省立医院中心室验室,250021 [3]山东省枣庄市立医院

出　　处：《中国药物与临床》2004年第11期837-839,共3页Chinese Remedies & Clinics

基　　金：山东省科技厅基金资助项目(1999BB1DBA4)

摘　　要：目的观察黄芪对糖尿病大鼠心肌细胞间黏附分子(ICAM)-1、血管黏附分子(VCAM)-1表达的影响。方法链脲佐菌素(STZ)诱导糖尿病大鼠模型。实验分正常对照组(n=8)、糖尿病对照组(n=11)、黄芪组(n=11,4.2g·kg-1·d-1),用药8周。测血糖、体重、糖化血红蛋白(HbA1c)、心脏重量;透射电镜观察心肌细胞超微结构变化;免疫组织化学检测心肌ICAM-1、VCAM-1表达,图像分析软件对其结果进行半定量分析。结果糖尿病组心重指数明显高于正常组(P<0.05);与糖尿病组比较,黄芪组心重指数下降(P<0.05)。正常组大鼠心肌细胞器完好;糖尿病组心肌细胞内线粒体水肿、肌原纤维溶解、肌浆网扩张、肌纤维间水肿等变化;黄芪组大鼠心肌细胞肌原纤维排列整齐,肌节较完整,线粒体量多,偶见肌原纤维断裂及线粒体肿胀。免疫组织化学显示:与正常组比较,糖尿病组心肌ICAM-1、VCAM-1强着色(P<0.05),胞质均呈棕黄色颗粒强,心肌组织间微动脉壁、毛细血管基底膜有棕黄色物质沉积;而黄芪组呈中等度染色,毛细血管基底膜着色,ICAM-1、VCAM-1表达明显下降(P<0.05)。结论黄芪降低糖尿病大鼠心肌ICAM-1、VCAM-1表达,同时保护糖尿病心肌细胞超微结构。Objective To investigate the effect of radix astragali on expression of intercellular adhesion molecule (ICAM)-1(CD54) and vascular adhesion molecule (VCAM)-1(CD106) of the myocardium of diabetic rats. Methods Diabetic rats were induced by streptozotocin (STZ) and were divided into different groups: diabetic model group (DM group, n=11), diabetic model with treatment of radix astragali group (DA, n=11, 4.2 g·kg-1·d-1) and pair-control group with non-treatment (NC, n=8) . After an 8-week treatment, blood glucose, weight, HbA1c and heart weight were detected respectively. Electron microscopy observed the changes of myocardial ultrastructure. The expression of myocardium ICAM-1 and VCAM-1 was assessed by immunohistochemistry; positive integrated intensity was quantified by image-analysis software. Results Compared with pair-control group, the structure of myocardium in diabetic group was demolished, the expression of ICAM-1 and VCAM-1 showed strong up-regulation. But in radix astragali group, above items were ameliorated compared with diabetic group (P<0.05). Conclusion Radix astragali is able to reduce the expression of ICAM-1 and VCAM-1, protecting the ultrastructure of myocardium of diabetic rats.

关 键 词：黄芪属植物 糖尿病 心肌 粘黏分子 超微结构 

分 类 号：R285[医药卫生—中药学]